Influence of nitrogen sources on growth and fermentation performance of different wine yeast species during alcoholic fermentation

• Published in: Applied microbiology and biotechnology 2015-12, Vol.99 (23), p.10191-10207
• Main Authors: Kemsawasd, Varongsiri, Viana, Tiago, Ardö, Ylva, Arneborg, Nils
• Format: Article
• Language: English
• Subjects: alanine; alcoholic fermentation; Amino acids; Amino Acids - metabolism; Ammonium; ammonium sulfate; ammonium sulphate; Analysis; Applied Microbial and Cell Physiology; arginine; asparagine; aspartic acid; Biomedical and Life Sciences; Biotechnology; Ethanol; Ethanol - metabolism; ethanol production; Fermentation; Food science; Glucose; glutamine; Hanseniaspora uvarum; Influence; isoleucine; Life Sciences; Metschnikowia pulcherrima; Microbial Genetics and Genomics; Microbiology; Nitrogen; Nitrogen - metabolism; Nitrogen sources; Physiology; Saccharomyces cerevisiae; serine; Studies; Sulfates; Torulaspora delbrueckii; valine; Vitaceae; Wine - microbiology; wine yeasts; Wines; Yeast; Yeasts; Yeasts - growth & development; Yeasts - metabolism
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-015-6835-3

In this study, the influence of twenty different single (i.e. 19 amino acids and ammonium sulphate) and two multiple nitrogen sources (N-sources) on growth and fermentation (i.e. glucose consumption and ethanol production) performance of Saccharomyces cerevisiae and of four wine-related non-Saccharomyces yeast species (Lachancea thermotolerans, Metschnikowia pulcherrima, Hanseniaspora uvarum and Torulaspora delbrueckii) was investigated during alcoholic fermentation. Briefly, the N-sources with beneficial effects on all performance parameters (or for the majority of them) for each yeast species were alanine, arginine, asparagine, aspartic acid, glutamine, isoleucine, ammonium sulphate, serine, valine and mixtures of 19 amino acids and of 19 amino acids plus ammonium sulphate (for S. cerevisiae), serine (for L. thermotolerans), alanine (for H. uvarum), alanine and asparagine (for M. pulcherrima), arginine, asparagine, glutamine, isoleucine and mixture of 19 amino acids (for T. delbrueckii). Furthermore, our results showed a clear positive effect of complex mixtures of N-sources on S. cerevisiae and on T. delbrueckii (although to a lesser extent) as to all performance parameters studied, whereas for L. thermotolerans, H. uvarum and M. pulcherrima, single amino acids affected growth and fermentation performance to the same extent as the mixtures. Moreover, we found groups of N-sources with similar effects on the growth and/or fermentation performance of two or more yeast species. Finally, the influences of N-sources observed for T. delbrueckii and H. uvarum resembled those of S. cerevisiae the most and the least, respectively. Overall, this work contributes to an improved understanding of how different N-sources affect growth, glucose consumption and ethanol production of wine-related yeast species under oxygen-limited conditions, which, in turn, may be used to, e.g. optimize growth and fermentation performance of the given yeast upon N-source supplementation during wine fermentations.