Improvement of RT-PCR Based Detection of Two Closteroviruses Associated with Little Cherry Disease in Sweet Cherries

The increasing availability of nucleotide sequence data from plant pathogenic fruit tree viruses led recently to the development of nucleic‐acid‐based detection methods as a routine tool for diagnosis. We present a simplification and modification of the previously described test procedure for the tw...

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Bibliographic Details
Published in:Journal of phytopathology 2004-02, Vol.152 (2), p.65-68
Main Authors: Rybak, M., Kountrias, A., Eppler, A., Jelkmann, W., Heinze, C., Adam, G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Closterovirus
detection
diagnosis
Fundamental and applied biological sciences. Psychology
PCR
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
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Summary:The increasing availability of nucleotide sequence data from plant pathogenic fruit tree viruses led recently to the development of nucleic‐acid‐based detection methods as a routine tool for diagnosis. We present a simplification and modification of the previously described test procedure for the two closteroviruses Little cherry virus 1 and 2 (LChV‐1, LChV‐2). For LChV‐1, a single tube reaction reduced unspecific amplification that led to false positive results. For the detection of LChV‐2, a primer set was developed for the local isolates of the area ‘Altes Land’ in the northern part of Germany. To determine the optimal sampling conditions for a reliable detection of both viruses different types of tissue (bark, bud, leaves) were tested in monthly intervals.
ISSN:0931-1785
1439-0434
DOI:10.1046/j.1439-0434.2003.00793.x