Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi

An antifungal dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The p...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-01, Vol.82, p.830-836
Main Authors: Xing, Ke, Shen, Xiaoqiang, Zhu, Xiao, Ju, Xiuyun, Miao, Xiangmin, Tian, Jun, Feng, Zhaozhong, Peng, Xue, Jiang, Jihong, Qin, Sheng
Format: Article
Language:English
Subjects:
Antifungal activity
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Chitosan
Chitosan - chemistry
Chitosan - pharmacology
DNA, Intergenic
Fatty Acids - chemistry
Fatty Acids - metabolism
Fungi - classification
Fungi - drug effects
Fungi - genetics
Fungi - metabolism
Microbial Sensitivity Tests
Mycelium - drug effects
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Particle Size
Phylogeny
Plant Diseases - microbiology
Plant pathogenic fungi
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Summary:An antifungal dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. Under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. The particle size of nanoparticles was around 296.962nm. Transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. Mycelium growth experiment demonstrated that Nigrospora sphaerica, Botryosphaeria dothidea, Nigrospora oryzae and Alternaria tenuissima were chitosan-sensitive, while Gibberella zeae and Fusarium culmorum were chitosan-resistant. The antifungal index was increased as the concentration of nanoparticles increased for chitosan-sensitive fungi. Fatty acid analyses revealed that plasma membranes of chitosan-sensitive fungi were shown to have lower levels of unsaturated fatty acid than chitosan-resistant fungi. Phylogenetic analysis based on ITS gene sequences indicated that two chitosan-resistant fungi had a near phylogenetic relationship. Results showed that O-chitosan nanoparticles could be a useful alternative for controlling pathogenic fungi in agriculture.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2015.09.074