Production of pullulan from raw potato starch hydrolysates by a new strain of Auerobasidium pullulans

In the present study, hydrolysis of potato starch with marine cold-adapted α-amylase and pullulan production from the hydrolysates by a new strain of Auerobasidium pullulans isolated from sea mud were conducted. The hydrolysis conditions were optimized as follows: reaction time 2h, pH 6.5, temperatu...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-01, Vol.82, p.740-743
Main Authors: Wu, Shengjun, Lu, Mingsheng, Chen, Jing, Fang, Yaowei, Wu, Leilei, Xu, Yan, Wang, Shujun
Format: Article
Language:English
Subjects:
alpha-Amylases
Ascomycota - metabolism
Chromatography, High Pressure Liquid
Fermentation
Glucans - biosynthesis
Glucans - isolation & purification
Hydrogen-Ion Concentration
Hydrolysis
Pullulan production
Solanum tuberosum - chemistry
Starch
Starch - chemistry
Starch - metabolism
Temperature
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Summary:In the present study, hydrolysis of potato starch with marine cold-adapted α-amylase and pullulan production from the hydrolysates by a new strain of Auerobasidium pullulans isolated from sea mud were conducted. The hydrolysis conditions were optimized as follows: reaction time 2h, pH 6.5, temperature 20°C, and α-amylase amount 12U/g. Under these optimum hydrolysis conditions, the DE value of the potato starch hydrolysates reached to 49.56. The potato starch hydrolysates consist of glucose, maltose, isomaltose, maltotriose, and trace of other maltooligosaccharides with degree of polymerization ranged 4–7. The maximum production of pullulan at 96h from the hydrolysate of potato starch was 36.17g/L, which was higher than those obtained from glucose (22.07g/L, p
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2015.09.075