Sepsis induced denervation-like changes at the neuromuscular junction

• Published in: The Journal of surgical research 2016-02, Vol.200 (2), p.523-532
• Main Authors: Liu, Li, PhD, Xie, Fei, PhD, Wei, Ke, MD, Hao, Xue-Chao, PhD, Li, Ping, PhD, Cao, Jun, MD, Min, Su, MD
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - metabolism; Demyelinating Diseases - metabolism; Demyelinating Diseases - physiopathology; Denervation; Electromyography; Male; Muscle denervation; Neuromuscular junction; Neuromuscular Junction - metabolism; Neuromuscular Junction - physiopathology; Nicotinic; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors; Sepsis; Sepsis - metabolism; Sepsis - physiopathology; Surgery; Tibial Nerve - metabolism; Tibial Nerve - physiopathology; Tibial Nerve - surgery
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2015.09.012

Abstract Background The aim of this study was to determine the functional and biochemical changes at the neuromuscular junction (NMJ) induced by sepsis. Materials and methods Male Sprague–Dawley rats were divided into three groups as follows: control, denervation, and sepsis. The rats were subjected to cecal ligation and puncture (CLP) or tibias nerve transection. NMJ function and the area of end plates were assessed, and the protein level of acetylcholine receptors and axonal neuregulin-1 was evaluated on postoperative days 1, 7, and 14. Results In the control group, the amplitude of compound muscle action potential (CMAP) was 16.51 ± 2.53 mV. In the sepsis group, the amplitude of CMAP decreased, and duration was prolonged on postoperative days 7 and 14 ( P