Brain tissue oxygen evaluation by wireless near-infrared spectroscopy

• Published in: The Journal of surgical research 2016-02, Vol.200 (2), p.669-675
• Main Authors: Wang, Che-Chuan, MD, Kuo, Jinn-Rung, MD, PhD, Chen, Yu-Chih, BS, Chio, Chung-Ching, MD, Wang, Jhi-Joung, MD, PhD, Lin, Bor-Shyh, PhD
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - metabolism; Brain - metabolism; Brain Injuries - metabolism; Deoxyhemoglobin; Male; Monitoring, Physiologic; Near-infrared spectroscopy; Oxygen - metabolism; Oxyhemoglobin; Partial pressure of oxygen in brain tissue; Random Allocation; Rats; Rats, Sprague-Dawley; Spectroscopy, Near-Infrared - methods; Surgery; Traumatic brain injury; Wireless Technology
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2015.10.005

Abstract Background Monitoring the partial pressure of oxygen in brain tissue (PbtO2 ) is an important tool for traumatic brain injury (TBI) but is invasive and inconvenient for real time monitoring. Near-infrared spectroscopy (NIRS), which can monitor hemoglobin parameters in the brain tissue, has been used widely as a noninvasive tool for assessing cerebral ischemia and hypoxia. Therefore, it may have the potential as a noninvasive tool for estimating the change of PbtO2 . In this study, a novel wireless NIRS system was designed to monitor hemoglobin parameters of rat brains under different impact strengths and was used to estimate the change of PbtO2 noninvasively in TBI. Materials and methods The proposed wireless NIRS system and a PbtO2 monitoring system were used to monitor the oxygenation of rat brains under different impact strengths. Rats were randomly assigned to four different impact strength groups (sham, 1.6 atm, 2.0 atm, and 2.4 atm; n  = 6 per group), and the relationships of concentration changes in oxyhemoglobin (HbO2 ), deoxyhemoglobin (HbR), and total hemoglobin (HbT), and PbtO2 during and after TBI with different impact strengths were investigated. Triphenyltetrazolium chloride (TTC) staining was also used to evaluate infarction volume. Results Concentration changes in HbO2 , HbR, and HbT dropped immediately after the impact, increased gradually, and then became stable. Changes in PbtO2 had a similar tendency with the hemoglobin parameters. There was significant correlation between changes in PbtO2 and HbO2 (correlation = 0.76) but not with changes in HbR (correlation = 0.06). In triphenyltetrazolium chloride staining, the infarction volume was highly but negatively associated with oxygen-related parameters like PbtO2 and HbO2. Conclusions Changes in HbO2 under TBI was highly and positively correlated with changes in PbtO2 . By using the relative changes in HbO2 as a reference parameter, the proposed wireless NIRS system may be developed as a noninvasive tool for estimating the change of PbtO2 in brain tissue after TBI.