Hydrogen peroxide centrally attenuates hyperosmolarity-induced thirst and natriuresis

• Published in: Neuroscience letters 2016-01, Vol.610, p.129-134
• Main Authors: Zanella, Regis C., Melo, Mariana Rosso, Furuya, Werner Issao, Colombari, Eduardo, Menani, José V., Colombari, Débora Simões Almeida
• Format: Article
• Language: English
• Subjects: Animals; Cations, Monovalent; Drinking - drug effects; Eating - drug effects; Hydrogen Peroxide - administration & dosage; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - pharmacology; Hypertonic saline; Injections, Intraventricular; Male; Natriuresis - drug effects; Osmolar Concentration; Osmoreceptors; Potassium - urine; Rats, Sprague-Dawley; Reactive oxygen species; Renal excretion; Sodium - urine; Sodium Chloride - pharmacology; Sucrose; Thirst - drug effects; Water intake
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2015.10.067

•Intragastric hypertonic NaCl simulates osmotically active substances ingestion.•Intragastric hypertonic NaCl induces thirst, natriuresis and diuresis.•H2O2 in the brain may modulate behavioral and renal responses.•Central H2O2 decreased hypertonic NaCl-induced thirst and natriuresis.•Central H2O2 also decreased meal-associated water intake. Intragastric hypertonic NaCl that simulates the ingestion of osmotically active substances by food intake induces thirst, vasopressin and oxytocin release, diuresis and natriuresis. Reactive oxygen species (ROS) produced endogenously in central areas may act modulating autonomic and behavioral responses. In the present study, we investigated the effects of H2O2 injected centrally on water intake and renal responses induced by increasing plasma osmolality with intragastric (ig) administration of 2M NaCl (2ml/rat). Male Holtzman rats (280–320g) with stainless steel cannula implanted in the lateral ventricle (LV) were used. Injections of H2O2 (2.5μmol/1μl) into the LV reduced ig 2M NaCl-induced water intake (3.1±0.7, vs. PBS: 8.6±1.0ml/60min, p