Naringin protects human adipose-derived mesenchymal stem cells against hydrogen peroxide-induced inhibition of osteogenic differentiation

• Published in: Chemico-biological interactions 2015-12, Vol.242, p.255-261
• Main Authors: Wang, Lei, Zhang, Yu-Ge, Wang, Xiu-Mei, Ma, Long-Fei, Zhang, Yuan-Min
• Format: Article
• Language: English
• Subjects: Adipose Tissue - cytology; Cell Differentiation - drug effects; Cell Survival - drug effects; Cytoprotection - drug effects; Flavanones - pharmacology; Human adipose-derived mesenchymal stem cells; Humans; Hydrogen Peroxide - adverse effects; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Naringin; Osteoblasts - cytology; Osteoblasts - drug effects; Osteogenesis - drug effects; Osteogenic differentiation; Oxidative stress; Oxidative Stress - drug effects; Wnt signaling; Wnt Signaling Pathway - drug effects
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2015.10.010

Extensive evidence indicates that oxidative stress plays a pivotal role in the development of osteoporosis. We show that naringin, a natural antioxidant and anti-inflammatory compound, effectively protects human adipose-derived mesenchymal stem cells (hADMSCs) against hydrogen peroxide (H2O2)-induced inhibition of osteogenic differentiation. Naringin increased viability of hAMDSCs and attenuated H2O2-induced cytotoxicity. Naringin also reversed H2O2-induced oxidative stress. Oxidative stress induced by H2O2 inhibits osteogenic differentiation by decreasing alkaline phosphatase (ALP) activity, calcium content and mRNA expression levels of osteogenesis marker genes RUNX2 and OSX in hADMSCs. However, addition of naringin leads to a significant recovery, suggesting the protective effects of naringin against H2O2-induced inhibition of osteogenic differentiation. Furthermore, the H2O2-induced decrease of protein expressions of β-catenin and clyclin D1, two important transcriptional regulators of Wnt-signaling, was successfully rescued by naringin treatment. Also, in the presence of Wnt inhibitor DKK-1, naringin is no longer effective in stimulating ALP activity, increasing calcium content and mRNA expression levels of RUNX2 and OSX in H2O2-exposed hADMSCs. These data clearly demonstrates that naringin protects hADMSCs against oxidative stress-induced inhibition of osteogenic differentiation, which may involve Wnt signaling pathway. Our work suggests that naringin may be a useful addition to the treatment armamentarium for osteoporosis and activation of Wnt signaling may represent attractive therapeutic strategy for the treatment of degenerative disease of bone tissue. •Naringin protects hADMSCs against H2O2-induced inhibition of differentiation.•Naringin increased viability of hAMDSCs and attenuated H2O2-induced cytotoxicity.•Naringin also reversed H2O2-induced oxidative stress.