Development of a PAN-Specific, Affinity-Purified Anti-acetylated Lysine Antibody for Detection, Identification, Isolation, and Intracellular Localization of Acetylated Protein

Acetylation on the lysine residue is an important event of posttranslational modification of proteins. In this study, we developed a simple method to produce and to affinity purify the specific anti-acetylated lysine polyclonal antibody, which is useful for the detection, identification, isolation,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2005-01, Vol.26 (1), p.13-23
Main Authors: Qiang, Liping, Xiao, Hao, Campos, Eric I., Ho, Vincent C., Li, Gang
Format: Article
Language:English
Subjects:
Acetylation
Affinity purification
Animals
Anti-AcK antibody
Antibodies - immunology
Antibodies - isolation & purification
Antibody Affinity
Antibody Specificity
Cattle
Chromatography, Affinity
ELISA
Hemocyanins - chemistry
Hemocyanins - immunology
Hemocyanins - isolation & purification
Histones - chemistry
Histones - immunology
Histones - metabolism
Immunohistochemistry
Immunoprecipitation
Lysine - chemistry
Lysine - immunology
Proteins - analysis
Proteins - chemistry
Proteins - immunology
Proteins - isolation & purification
Rabbits
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - immunology
Serum Albumin, Bovine - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acetylation on the lysine residue is an important event of posttranslational modification of proteins. In this study, we developed a simple method to produce and to affinity purify the specific anti-acetylated lysine polyclonal antibody, which is useful for the detection, identification, isolation, and intracellular localization of acetylated proteins on the lysine residues. We utilized the chemically acetylated hemocyanin of keyhole limpets (KLH) as an immunogen to raise the immune serum and to isolate the population of the acetylated lysine specific antibody using the immobilized acetylated lysine as immunoaffinity-ligand. The isolated antibody was tested to be useful for ELISA, immunoblotting detection, immunofluorescent localization, and affinity isolation of the acetylated proteins.
ISSN:1532-1819
1532-4230
DOI:10.1081/IAS-200041153