Dual Renin Gene Targeting by Cre-Mediated Interchromosomal Recombination

This study describes a new approach to targeting clustered genes. Our study began with the establishment of two lines of mice carrying different mutations in either Ren1 or Ren2. These two genes, both encoding renin, span over 40 kb in tandem on chromosome 1. Each gene was mutated by gene targeting...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2000-03, Vol.64 (2), p.127-131
Main Authors: Matsusaka, Taiji, Kon, Valentina, Takaya, Junji, Katori, Hideyuki, Chen, Xian-mei, Miyazaki, Jun-ichi, Homma, Toshio, Fogo, Agnes, Ichikawa, Iekuni
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
chromosome 1
Chromosomes, Human, Pair 1
Female
Fundamental and applied biological sciences. Psychology
Gene Targeting
Genes, Reporter
Genetic Engineering
Genetic Vectors
Hemagglutinins, Viral - biosynthesis
Hemagglutinins, Viral - genetics
Humans
Immunohistochemistry
Integrases - metabolism
Kidney - chemistry
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Molecular and cellular biology
Molecular genetics
Multigene Family - genetics
Mutagenesis, Site-Directed
Mutagenesis. Repair
Recombination, Genetic
Ren1 gene
Ren2 gene
Renin - genetics
Viral Proteins
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Summary:This study describes a new approach to targeting clustered genes. Our study began with the establishment of two lines of mice carrying different mutations in either Ren1 or Ren2. These two genes, both encoding renin, span over 40 kb in tandem on chromosome 1. Each gene was mutated by gene targeting to contain loxP sites. These two mutants and Cre transgenic mice were mated to produce offspring carrying the mutant Ren1 and Ren2 genes, as well as the Cre transgene concurrently. Initially, two mutant Ren genes were located on separate chromosomes. Southern analysis of mice from the second generation revealed that the mutant Ren1 and Ren2 were interchromosomally recombined at the loxP sites to produce a new dually mutated allele on the chromosome at the rate of 9.6% (7/73). Thus, interchromosomal recombination can be efficiently programmed by mating as designed using the Cre–loxP system.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2000.6113