Cloning, functional expression and partial characterization of the glucose kinase from Renibacterium salmoninarum

The complete glcK gene from the fish pathogen Renibacterium salmoninarum, encoding a glucose kinase, was analyzed and expressed. The partial characterization of the recombinant enzyme confirmed that it belongs to a group of glucose kinases involved in carbon catabolite repression. Multiple sequence...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2000-05, Vol.186 (1), p.97-101
Main Authors: Concha, Margarita I, León, Gloria
Format: Article
Language:English
Subjects:
Consensus sequence
Glucose kinase
N-Ethylmaleimide
Renibacterium salmoninarum
ROK family
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The complete glcK gene from the fish pathogen Renibacterium salmoninarum, encoding a glucose kinase, was analyzed and expressed. The partial characterization of the recombinant enzyme confirmed that it belongs to a group of glucose kinases involved in carbon catabolite repression. Multiple sequence alignments were used to deduce a new consensus sequence for this family of bacterial proteins, characterized by several conserved Cys residues. This sequence was more specific and allowed the detection of the first eukaryotic protein of this family. The recombinant enzyme was inhibited by N-ethylmaleimide and the substrates protected the enzyme from this inhibition, suggesting the presence of Cys residues in or close to the active site.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(00)00124-5