Development of microsatellite markers for Carapa guianensis (Aublet), a tree species from the Amazon forest

Carapa guianensis is a timber species found in Central America and the north of South America. We have developed microsatellite primers which will allow analysis of gene flow and population genetic structure in natural populations of this tree species. Polymorphism of five microsatellite loci was ev...

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Bibliographic Details
Published in:Molecular ecology notes 2005-03, Vol.5 (1), p.33-34
Main Authors: Vinson, C.C, Azevedo, V.C.R, Sampaio, I, Ciampi, A.Y
Format: Article
Language:English
Subjects:
alleles per locus
Amazon
Carapa guianensis
DNA primers
forest trees
genetic markers
heterozygosity
loci
microsatellite repeats
microsatellites
molecular sequence data
nucleotide sequences
polymerase chain reaction
tropical tree
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Summary:Carapa guianensis is a timber species found in Central America and the north of South America. We have developed microsatellite primers which will allow analysis of gene flow and population genetic structure in natural populations of this tree species. Polymorphism of five microsatellite loci was evaluated using a total of 12 adult trees from a natural population. An average of 4.2 alleles per locus was detected, and expected heterozygosity ranging from 0.397 to 0.806. These loci are being used for genetic population analysis in a managed forest in the state of Pará, in Amazonian Brazil, as part of the Dendrogene project.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00821.x