Characterisation of rotaviruses from children treated at a London Hospital during 1996 : Emergence of strains G9P2A[6] and G3P2A[6]

Rotavirus strains from 171 patients treated in 1996 at a children's hospital in London were characterised. Use of a panel of typing monoclonal antibodies for serotypes G1-4 identified 105 (61%) of the strains. The majority, 90 strains (86%), were serotype G1. Characterisation of G (VP7) and P (...

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Bibliographic Details
Published in:Journal of medical virology 2000-05, Vol.61 (1), p.150-154
Main Authors: CUBITT, W. D, STEELE, A. D, ITURRIZA, M
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
British Isles, England, London
Child
Child, Preschool
Continental Population Groups
Female
Genotype
Human viral diseases
Humans
Immunoenzyme Techniques
Infant
Infectious diseases
London - epidemiology
Male
Medical sciences
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - immunology
Rotavirus Infections - diagnosis
Rotavirus Infections - ethnology
Rotavirus Infections - virology
Serotyping
Viral diseases
Viral diseases of the digestive system
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Summary:Rotavirus strains from 171 patients treated in 1996 at a children's hospital in London were characterised. Use of a panel of typing monoclonal antibodies for serotypes G1-4 identified 105 (61%) of the strains. The majority, 90 strains (86%), were serotype G1. Characterisation of G (VP7) and P (VP4) types using reverse transcription-polymerase chain reaction was more efficient, and 167 of 171 (98%) of the strains were identified this way. The predominant strains were G1P1A[8] (55%) and G4P1A[8] (17%), which are prevalent throughout the world; however, a significant number of cases were associated with genotypes not recorded previously in the United Kingdom. There were 21 (13%) cases associated with G9P2A[6] and 11 (6%) cases associated with G3P2A[6]. The majority (seven of 10) cases of infection in children older than 3 years of age were caused by these two genotypes. A majority (15/21) of G9P2A[6] strains were recovered from children admitted to the hospital, and five children were sufficiently dehydrated to necessitate intravenous rehydration.
ISSN:0146-6615
1096-9071
DOI:10.1002/(SICI)1096-9071(200005)61:1<150::AID-JMV24>3.0.CO;2-W