Benzene analysis in workplace air using an FIA-based bacterial biosensor

• Published in: Biosensors & bioelectronics 2005-04, Vol.20 (10), p.2089-2096
• Main Authors: Lanyon, Yvonne H., Marrazza, Giovanna, Tothill, Ibtisam E., Mascini, Marco
• Format: Article
• Language: English
• Subjects: Air Pollutants, Occupational - analysis; Air Pollutants, Occupational - pharmacology; Bacterial biosensor; Benzene; Benzene - analysis; Benzene - pharmacology; Biological and medical sciences; Biological Assay - instrumentation; Biological Assay - methods; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Dissolved oxygen electrode; Dose-Response Relationship, Drug; Equipment Design; Equipment Failure Analysis; Flow injection analysis; Flow Injection Analysis - instrumentation; Flow Injection Analysis - methods; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Oxygen Consumption - drug effects; Oxygen Consumption - physiology; Pseudomonas putida; Pseudomonas putida - drug effects; Pseudomonas putida - metabolism; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2004.08.034

A bacterial biosensor based on flow injection analysis (FIA) has been developed for the determination of benzene in workplace air samples. Benzene can be used by the bacteria Pseudomonas putida ML2 as a sole carbon source, and its aerobic degradation can be measured using a dissolved oxygen electrode. The bacterial cells were immobilised between two cellulose acetate membranes and fixed onto a Clark dissolved oxygen probe, which was inserted into a custom-made flow cell. The applicability of the biosensor for the analysis of air samples containing benzene was investigated. Air samples were collected from a controlled exposure room using charcoal adsorption tubes, and benzene extracted with solvent desorption using dimethylformamide (DMF). The biosensor displayed a linear detection range between 0.025 and 0.15 mM benzene based on standard solutions containing a maximum of 2% DMF, with a response time of 6 min. This linear detection range allows the analysis of air containing between 3 and 16 ppm benzene based on a 60-min sampling period. DMF proved to be compatible for use with the biosensor, causing minimal interference with the sensor response and causing no toxic effects on the bacterial cells. The FIA system was easily transported to an in situ location, and a correlation was obtained between the biosensor and gas chromatography (GC) results for the preliminary air samples investigated. Moreover, the biosensor displayed no interference to other benzene related compounds in the BTEX range. The results from this work have shown that the biosensor has potential applications for the analysis of benzene in workplace air samples, with the added advantages over the conventional GC methods of low operation costs, ease of use, and portability for in situ measurements.