Increased expression of copine VI in patients with refractory epilepsy and a rat model

Abstract Copine VI (CPNE6) is a member of copines family, a calcium-dependent phospholipids-binding protein group found in many diverse eukaryotic organisms. Although earlier studies have shown that CPNE6 is almost exclusively expressed in brain, the exact biological functions remain unclear. The pu...

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Bibliographic Details
Published in:Journal of the neurological sciences 2016-01, Vol.360, p.30-36
Main Authors: Zhu, Binglin, Zha, Jingsi, Long, Yan, Hu, Xiaotong, Chen, Guojun, Wang, Xuefeng
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Brain - metabolism
Carrier Proteins - metabolism
Child
Copine VI (CPNE6)
Disease Models, Animal
Drug Resistant Epilepsy - metabolism
Drug Resistant Epilepsy - surgery
Epilepsy, Temporal Lobe - metabolism
Epilepsy, Temporal Lobe - surgery
Female
Humans
Male
Membrane Proteins - metabolism
Nerve Tissue Proteins - metabolism
Neurology
Neurons - metabolism
Patients
Rat model
Rats
Rats, Sprague-Dawley
Refractory epilepsy
Spontaneous recurrent seizures
Young Adult
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Summary:Abstract Copine VI (CPNE6) is a member of copines family, a calcium-dependent phospholipids-binding protein group found in many diverse eukaryotic organisms. Although earlier studies have shown that CPNE6 is almost exclusively expressed in brain, the exact biological functions remain unclear. The purpose of this study is to explore the relationship between epilepsy and CPNE6 expression. In present study, we investigated the expression pattern and distribution of CPNE6 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic rat model by quantitative real-time PCR, Western blot and immunofluorescence. The results showed that the expression of CPNE6 increased remarkably in epileptic patients and in experimental epileptic rats. Double immunofluorescence labeling studies have revealed that CPNE6 protein is mainly expressed in neurons, demonstrated by co-localization with the dendritic marker, MAP2. Our results are the first to indicate that the abnormal expression of the CPNE6 in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy.
ISSN:0022-510X
1878-5883
DOI:10.1016/j.jns.2015.11.041