What is the current optimal fat grafting processing technique? A systematic review

Abstract Background With the advents of new processing techniques and new graft survival theories in fat grafting, the question is: Which processing technique is of preference? This study systematically reviewed literature regarding current techniques for processing fat grafts. Methods PubMed, Embas...

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Published in:Journal of cranio-maxillo-facial surgery 2016-01, Vol.44 (1), p.45-55
Main Authors: Tuin, A. Jorien, Domerchie, Patrick N, Schepers, Rutger H, Willemsen, Joep C.N, Dijkstra, Pieter U, Spijkervet, Fred K.L, Vissink, Arjan, Jansma, Johan
Format: Article
Language:English
Subjects:
Adipocytes
Adipose derived stromal/stem cells
Adipose Tissue - transplantation
Animals
Centrifugation
Dentistry
Fat graft
Graft Survival
Humans
Processing
Stromal Cells
Surgery
Systematic review
Viability
Volume
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Summary:Abstract Background With the advents of new processing techniques and new graft survival theories in fat grafting, the question is: Which processing technique is of preference? This study systematically reviewed literature regarding current techniques for processing fat grafts. Methods PubMed, Embase, Cinahl, and Cochrane databases were searched until August 2015. Studies comparing different fat grafting processing techniques were included. Outcomes were viability of adipocytes, number of adipose-derived stromal/stem cells (ASC) and growth factors in vitro, volume and quality of the graft in animal studies, and satisfaction and volume retention in human studies. Results Thirty-five studies were included. Adipocyte viability and ASC numbers were the best using the gauze/towel technique (permeability principle) compared to centrifugation. With regard to centrifugation, the pellet contained more ASCs compared to the middle layer. The animal studies' and patients' satisfaction results were not distinctive. The only study assessing volume retention in humans showed that a wash filter device performed significantly better than centrifugation. Conclusion In this study, processing techniques using permeability principles proved superior to centrifugation (reinforced gravity principle) regarding viability and ASC number. Due to the variety in study characteristics and reported outcome variables, however, none of the processing techniques in this study demonstrated clinical evidence of superiority.
ISSN:1010-5182
1878-4119
DOI:10.1016/j.jcms.2015.10.021