Plant regeneration of three Opuntia genotypes used as human food

In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to i...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2005-02, Vol.80 (2), p.215-219
Main Authors: Garcia-Saucedo, P.A, Valdez-Morales, M, Valverde, M.E, Cruz-Hernandez, A, Paredes-Lopez, O
Format: Article
Language:English
Subjects:
benzyladenine
Biological and medical sciences
Biotechnology
buds
cacti and succulents
cladodes
culture media
dose response
edible cacti
Eukaryotic cell cultures
explants
Fundamental and applied biological sciences. Psychology
genetic variation
genotype
gibberellic acid
in vitro culture
in vitro regeneration
indole butyric acid
methodology
Methods. Procedures. Technologies
micropropagation
Miscellaneous
Murashige and Skoog medium
Opuntia
Opuntia ficus-indica
Plant cells and fungal cells
shoots
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Summary:In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 micromolar BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-004-9158-0