Exposure to work-related levels of swine dust up-regulates CD106 on human alveolar macrophages

• Published in: American journal of industrial medicine 2004-10, Vol.46 (4), p.378-380
• Main Authors: Hoffmann, Hans Jürgen, Iversen, Martin, Takai, Hisamitsu, Sigsgaard, Torben, Omland, Øyvind, Dahl, Ronald
• Format: Article
• Language: English
• Subjects: Air Pollutants, Occupational - metabolism; Air Pollution, Indoor - analysis; Animals; Biological and medical sciences; Bronchoalveolar Lavage Fluid; Bronchoscopes; CD106/VCAM1; Chemical and industrial products toxicology. Toxic occupational diseases; Dust - analysis; Endotoxins - analysis; human; Humans; Inorganic dusts (pneumoconiosises) and organic dusts (byssinosis etc.); lipopolysacchrides; Macrophages, Alveolar - cytology; Macrophages, Alveolar - metabolism; Medical sciences; monocyte/macrophage; Swine; swine confinement buildings; Toxicology; Up-Regulation; Vascular Cell Adhesion Molecule-1 - analysis
• ISSN: 0271-3586; 1097-0274
• DOI: 10.1002/ajim.20078

Background Exposure to dust from swine confinement buildings, causes an inflammatory response. Monocyte recruitment to the murine lung after instillation of lipopolysaccharide (LPS) has been shown to partially depend on CD106/VCAM1. We wanted to determine whether this can be confirmed in man. Methods Non‐naïve persons bearing personal air samples were exposed for 3 hr in a swine confinement building, and were bronchoscopied before and after exposure. Blood samples were taken at the time of bronchoscopy. Expression of adhesion molecules on leukocytes was investigated by flow cytometry in bronchoaveloar lavage (BAL) and blood. Results Expression of CD106 on alveolar macrophages, but not on neutrophils or T cells in BAL or blood, was up‐regulated in proportion to the amount of LPS that individuals were exposed. Conclusions This argues for requirement of CD106 during inflammatory recruitment of monocytes to the human lung. Am. J. Ind. Med. 46:378–380, 2004. © 2004 Wiley‐Liss, Inc.