SPARC modulates expression of extracellular matrix genes in human trabecular meshwork cells

• Published in: Acta ophthalmologica (Oxford, England) England), 2012-03, Vol.90 (2), p.e138-e143
• Main Authors: Wei, Hai-Ying, Liu, Jing-Lei, Lv, Bing-Jie, Xing, Lin, Fu, Shao-Ying
• Format: Article
• Language: English
• Subjects: acidic cysteine-rich secreted protein; Blotting, Western; Cells, Cultured; Collagen Type I - genetics; Collagen Type III - genetics; Fibronectins - genetics; Gene Expression Regulation - physiology; Gene Silencing - physiology; glaucoma; Humans; Osteonectin; real-time RT-PCR; Reverse Transcriptase Polymerase Chain Reaction; RNA interference; RNA, Messenger - metabolism; RNA, Small Interfering - genetics; Trabecular Meshwork - metabolism; Transfection; Tumor Suppressor Proteins - genetics; Western blot
• ISSN: 1755-375X; 1755-3768
• DOI: 10.1111/j.1755-3768.2011.02283.x

. Purpose:  To investigate the effects of secreted protein acidic and rich in cysteine (SPARC) on the expression of components of the extracellular matrix (ECM) in cultured human trabecular meshwork (TM) cells. Methods:  Cultured human trabecular cells were transfected with small interfering RNAs (siRNAs) specific for the human SPARC gene. Protein and mRNA expressions of fibronectin (FN) and the α1chains of collagen I and collagen III were quantified. Results:  After silencing of the SPARC gene by transfection of cells with SPARC siRNA, the expression of COL1A1 and COL3A1 mRNAs and proteins was significantly enhanced, as compared to that in the control group (all, p