Mass culture strategy for bacterial yeast co-culture for degradation of petroleum hydrocarbons in marine environment

In the present study a metabolically versatile co-culture with two Bacilli and one yeast strain was developed using enrichment culture techniques. The developed co-culture had affinity to degrade both aliphatic and aromatic fractions of petroleum crude oil. Degradation kinetics was established for d...

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Bibliographic Details
Published in:Marine pollution bulletin 2015-11, Vol.100 (1), p.191-199
Main Authors: Priya, Anchal, Mandal, Ajoy K., Ball, Andrew S., Manefield, Mike, Lal, Banwari, Sarma, Priyangshu M.
Format: Article
Language:English
Subjects:
Aliphatic and aromatic hydrocarbons
Bacillus - metabolism
Bacteria
Bioaugmentation
Biodegradation
Biodegradation, Environmental
Bioreactor
Bioreactors - microbiology
Candida - metabolism
Coculture Techniques
Hydrocarbons - metabolism
India
Marine
Mass-culture strategy
Microbial Consortia
Petroleum - metabolism
Petroleum Pollution
Seawater
Seawater - microbiology
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Summary:In the present study a metabolically versatile co-culture with two Bacilli and one yeast strain was developed using enrichment culture techniques. The developed co-culture had affinity to degrade both aliphatic and aromatic fractions of petroleum crude oil. Degradation kinetics was established for designing the fermentation protocol of the co-culture. The developed mass culture strategy led to achieve the reduction in surface tension (26dynescm−1 from 69 dynescm−1) and degradation of 67% in bench scale experiments. The total crude oil degradation of 96% was achieved in 4000l of natural seawater after 28days without adding any nutrients. The survival of the augmented co-culture was maintained (109cellsml−1) in contaminated marine environment. The mass culture protocol devised for the bioaugmentation was a key breakthrough that was subsequently used for pilot scale studies with 100l and 4000l of natural seawater for potential application in marine oil spills. •Development of co-culture with two bacteria and a yeast strain•Petroleum crude oil degradation in marine environment•Degradation of model aliphatic and aromatic hydrocarbons•Mass culture strategy for the developed co-culture in 150l bioreactor•Bioaugmentation: mesocosm 100l (Bay of Bengal) and Pilot Scale 4000l (Arabian Sea)
ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2015.08.050