Protease Producing Bacteria and Activity in Gut of Tiger Shrimp (Penaeus monodon)

Proteases, the group of enzyme with significant commercial value, was isolated from proteolytic bacteria available in gut of tiger shrimp (Penaeus monodon). The isolation of bacteria from the gut of the collected shrimp was performed by serial dilution and plating method. Six bacterial isolates were...

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Bibliographic Details
Published in:Journal of fisheries and aquatic science 2015-11, Vol.10 (6), p.489-489
Main Authors: Bhowmik, Shuva, Islam, Sumaiya, Ahmed, Monzur Morshed, Hossain, M Belal, Hossain, Md Abul
Format: Article
Language:English
Subjects:
Bacteria
Decapoda
Penaeus monodon
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Summary:Proteases, the group of enzyme with significant commercial value, was isolated from proteolytic bacteria available in gut of tiger shrimp (Penaeus monodon). The isolation of bacteria from the gut of the collected shrimp was performed by serial dilution and plating method. Six bacterial isolates were screened out on the basis of their formation of zone of casein hydrolysis on skim milk agar. Among the six isolates, three isolates were found gram positive and the rest three isolates were found gram negative. Protease activity of the isolates was determined both qualitatively and quantitatively. The effect of pH and NaCl concentration on the growth and protease production of the isolates were also studied by assaying protease activity at different pH range and NaCl concentrations. The isolates exhibited maximum protease production at varying pH and NaCl concentrations. The data showed potentiality of the bacterial isolates to be a useful source of industrial protease.
ISSN:1816-4927
1996-0751
DOI:10.3923/jfas.2015.489.500