Elicitation of HIV-1 neutralizing antibodies by presentation of 4E10 and 10E8 epitopes on Norovirus P particles

Highlights • 4E10 or 10E8 epitopes inserted into the P domain of Norovirus P particle could induce highly humoral immune responses in Balb/c mice and guinea pigs. • 4E10 or 10E8 epitopes presented on three loops of P domain resulted in better antibody binding levels and neutralizing antibody titers...

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Bibliographic Details
Published in:Immunology letters 2015-12, Vol.168 (2), p.271-278
Main Authors: Yu, Yongjiao, Fu, Lu, Shi, Yuhua, Guan, Shanshan, Yang, Lan, Gong, Xin, Yin, He, He, Xiaoqiu, Liu, Dongni, Kuai, Ziyu, Shan, Yaming, Wang, Song, Kong, Wei
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Neutralizing - immunology
Broadly neutralizing antibody
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Female
Guinea Pigs
HIV Antibodies - immunology
HIV-1 - immunology
HIV-1 vaccine
Human immunodeficiency virus
Human immunodeficiency virus 1
Immunization - methods
Mice, Inbred BALB C
Microscopy, Electron, Transmission
MPER
Norovirus
Norovirus - immunology
Norovirus - ultrastructure
Norovirus P particle
Viral Vaccines - immunology
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Summary:Highlights • 4E10 or 10E8 epitopes inserted into the P domain of Norovirus P particle could induce highly humoral immune responses in Balb/c mice and guinea pigs. • 4E10 or 10E8 epitopes presented on three loops of P domain resulted in better antibody binding levels and neutralizing antibody titers than in loop 2. • The α-helix content was increased obviously when 4E10 or 10E8 epitopes inserted into three loops of P domain than in loop 2, which increased the generation of neutralizing activities.
ISSN:0165-2478
1879-0542
DOI:10.1016/j.imlet.2015.10.003