Antioxidant enzymes activities of Burkholderia spp. strains—oxidative responses to Ni toxicity

• Published in: Environmental science and pollution research international 2015-12, Vol.22 (24), p.19922-19932
• Main Authors: Dourado, M. N, Franco, M. R, Peters, L. P, Martins, P. F, Souza, L. A, Piotto, F. A, Azevedo, R. A
• Format: Article
• Language: English
• Subjects: Agricultural production; Antioxidants; Antioxidants - metabolism; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Biodegradation, Environmental; Bioremediation; Biosorption; Burkholderia; Burkholderia - drug effects; Burkholderia - enzymology; Burkholderia - growth & development; Burkholderia - metabolism; catalase; DNA; DNA damage; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental impact; enzyme activity; Enzymes; Flowers & plants; Fungicides; Gene expression; Glutathione reductase; Glutathione transferase; glutathione-disulfide reductase; Groundwater; Heavy metals; Herbicides; isozymes; Lipids; Metabolism; Metabolites; Metal concentrations; Metals; microbial growth; Microorganisms; Mobility; Nickel; Nickel - toxicity; Oxidation-Reduction - drug effects; Oxidative stress; Oxidative Stress - drug effects; Pesticides; polluted soils; polyacrylamide gel electrophoresis; Proteins; Reactive oxygen species; Research Article; Soil contamination; Soil Microbiology; Soil microorganisms; Soil Pollutants - toxicity; Soil pollution; Species Specificity; Strains (organisms); Superoxide dismutase; Toxicity; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-015-5204-1

Increased agriculture production associated with intense application of herbicides, pesticides, and fungicides leads to soil contamination worldwide. Nickel (Ni), due to its high mobility in soils and groundwater, constitutes one of the greatest problems in terms of environmental pollution. Metals, including Ni, in high concentrations are toxic to cells by imposing a condition of oxidative stress due to the induction of reactive oxygen species (ROS), which damage lipids, proteins, and DNA. This study aimed to characterize the Ni antioxidant response of two tolerant Burkholderia strains (one isolated from noncontaminated soil, SNMS32, and the other from contaminated soil, SCMS54), by measuring superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione S-transferase (GST) activities. Ni accumulation and bacterial growth in the presence of the metal were also analyzed. The results showed that both strains exhibited different trends of Ni accumulation and distinct antioxidant enzymes responses. The strain from contaminated soil (SCMS54) exhibited a higher Ni biosorption and exhibited an increase in SOD and GST activities after 5 and 12 h of Ni exposure. The analysis of SOD, CAT, and GR by nondenaturing PAGE revealed the appearance of an extra isoenzyme in strain SCMS54 for each enzyme. The results suggest that the strain SCMS54 isolated from contaminated soil present more plasticity with potential to be used in soil and water bioremediation.