Enlargement of individual cellulose microfibrils in transgenic poplars overexpressing xyloglucanase

Holocellulose samples prepared from transgenic poplars overexpressing xyloglucanase had crystal widths of 3.2-3.5 nm as a result of the (2 0 0) plane, based on their X-ray diffraction patterns, and crystal widths were greater than those of the wild type (3.0 nm). Cellulose microfibril widths in the...

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Bibliographic Details
Published in:Journal of wood science 2011-02, Vol.57 (1), p.71-75
Main Authors: Yamamoto, Mako, Saito, Tsuguyuki, Isogai, Akira, Kurita, Manabu, Kondo, Teiji, Taniguchi, Toru, Kaida, Rumi, Baba, Keiichi, Hayashi, Takahisa
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cellulose
Cellulose fibril
Characterization and Evaluation of Materials
Crystals
Diffraction patterns
Disintegration
Enlargement
Image transmission
Life Sciences
Materials Science
Oxidation
TEMPO-mediated oxidation
Transgenic poplar
Transmission electron microscopy
Wood Science & Technology
X-ray diffraction
Xyloglucanase
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Summary:Holocellulose samples prepared from transgenic poplars overexpressing xyloglucanase had crystal widths of 3.2-3.5 nm as a result of the (2 0 0) plane, based on their X-ray diffraction patterns, and crystal widths were greater than those of the wild type (3.0 nm). Cellulose microfibril widths in the holocellulose samples were further determined from transmission electron microscopic (TEM) images of individualized fibrils prepared by 2,2,6,6-tetramethylpiperidine-1-oxy radical-mediated oxidation of the holocelluloses and the successive disintegration of the oxidized products in water. The TEM images also supported the finding that cellulose microfibril widths of transgenic poplars were larger than those of the wild type. The cellulose microfibril widths of transgenic poplars were approximately 6 nm, whereas those of the wild type were about 5 nm. However, such enlargement of cellulose microfibril widths could not be explained by the increased cellulose contents of the transgenic poplars alone.
ISSN:1435-0211
1611-4663
DOI:10.1007/s10086-010-1140-3