The cytosolic splicing variant of NELL2 inhibits PKC beta 1 in glial cells

NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-spe...

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Published in:Biochemical and biophysical research communications 2014-11, Vol.454 (3), p.459-464
Main Authors: Lee, Da Yong, Kim, Eunju, Lee, Young-Sun, Ryu, Hwani, Park, Jae-Yong, Hwang, Eun Mi
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container_title Biochemical and biophysical research communications
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creator Lee, Da Yong
Kim, Eunju
Lee, Young-Sun
Ryu, Hwani
Park, Jae-Yong
Hwang, Eun Mi
description NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKC beta isotypes and inhibits PKC beta 1 through direct binding to the N-terminal pseudosubstrate domain of PKC beta 1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKC beta 1 in glia.
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title The cytosolic splicing variant of NELL2 inhibits PKC beta 1 in glial cells
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