Ion-exchange vs reversed-phase chromatography for separation and determination of basic psychotropic drugs

Ion exchange chromatography, an alternative to reversed‐phase (RP) chromatography, is described in this paper. We aimed to obtain optimal conditions for the separation of basic drugs because silica‐based RP stationary phases show silanol effect and make the analysis of basic analytes hardly possible...

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Bibliographic Details
Published in:Biomedical chromatography 2015-11, Vol.29 (11), p.1700-1707
Main Authors: Petruczynik, Anna, Wróblewski, Karol, Deja, Michał, Waksmundzka-Hajnos, Monika
Format: Article
Language:English
Subjects:
Chromatography, Ion Exchange - methods
Chromatography, Reverse-Phase
Humans
IEC-HPLC
Limit of Detection
optimization
psychotropic drugs
Psychotropic Drugs - blood
Psychotropic Drugs - isolation & purification
quantitative analysis
Reproducibility of Results
RP-HPLC
Solid Phase Extraction
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Summary:Ion exchange chromatography, an alternative to reversed‐phase (RP) chromatography, is described in this paper. We aimed to obtain optimal conditions for the separation of basic drugs because silica‐based RP stationary phases show silanol effect and make the analysis of basic analytes hardly possible. The retention, separation selectivity, symmetry of peaks and system efficiency were examined in different eluent systems containing different types of buffers at acidic pH and with the addition of organic modifiers: methanol and acetonitrile. The obtained results reveal a large influence of the salt cation used for buffer preparation and the type of organic modifier on the retention behavior of the analytes. These results were also compared with those obtained on an XBridge C18 column. The obtained results demonstrated that SCX stationary phases can be successfully used as alternatives to C18 stationary phases in the separation of basic compounds. The most selective and efficient chromatographic systems were applied for the quantification of some psychotropic drugs in fortified human serum samples. Copyright © 2015 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3482