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The small GTPase MoYpt7 is required for membrane fusion in autophagy and pathogenicity of Magnaporthe oryzae
Summary Rab GTPases are required for vesicle–vacuolar fusion during vacuolar biogenesis in fungi. To date, little is known about the biological functions of the Rab small GTPase components in Magnaporthe oryzae. In this study, we investigated MoYpt7 of M. oryzae, a homologue of the small Ras‐like GT...
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Published in: | Environmental microbiology 2015-11, Vol.17 (11), p.4495-4510 |
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creator | Liu, Xiao-Hong Chen, Si-Miao Gao, Hui-Min Ning, Guo-Ao Shi, Huan-Bin Wang, Yao Dong, Bo Qi, Yao-Yao Zhang, Dong-Mei Lu, Guo-Dong Wang, Zong-Hua Zhou, Jie Lin, Fu-Cheng |
description | Summary
Rab GTPases are required for vesicle–vacuolar fusion during vacuolar biogenesis in fungi. To date, little is known about the biological functions of the Rab small GTPase components in Magnaporthe oryzae. In this study, we investigated MoYpt7 of M. oryzae, a homologue of the small Ras‐like GTPase Ypt7 in Saccharomyces cerevisiae. Cellular localization assays showed that MoYpt7 was predominantly localized to vacuolar membranes. Using a targeted gene disruption strategy, a ΔMoYPT7 mutant was generated that exhibited defects in mycelial growth and production of conidia. The conidia of the ΔMoYPT7 mutant were malformed and defective in the formation of appressoria. Consequently, the ΔMoYPT7 mutant failed to cause disease in rice and barley. Furthermore, the ΔMoYPT7 mutant showed impairment in autophagy, breached cell wall integrity, and higher sensitivity to both calcium and heavy metal stress. Transformants constitutively expressing an active MoYPT7 allele (MoYPT7‐CA, Gln67Leu) exhibited distinct phenotypes from the ΔMoYPT7 mutant. Expression of MoYPT7‐CA in MoYpt7 reduced pathogenicity and produced more appressoria‐forming single‐septum conidia. These results indicate that MoYPT7 is required for fungal morphogenesis, vacuole fusion, autophagy, stress resistance and pathogenicity in M. oryzae. |
doi_str_mv | 10.1111/1462-2920.12903 |
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Rab GTPases are required for vesicle–vacuolar fusion during vacuolar biogenesis in fungi. To date, little is known about the biological functions of the Rab small GTPase components in Magnaporthe oryzae. In this study, we investigated MoYpt7 of M. oryzae, a homologue of the small Ras‐like GTPase Ypt7 in Saccharomyces cerevisiae. Cellular localization assays showed that MoYpt7 was predominantly localized to vacuolar membranes. Using a targeted gene disruption strategy, a ΔMoYPT7 mutant was generated that exhibited defects in mycelial growth and production of conidia. The conidia of the ΔMoYPT7 mutant were malformed and defective in the formation of appressoria. Consequently, the ΔMoYPT7 mutant failed to cause disease in rice and barley. Furthermore, the ΔMoYPT7 mutant showed impairment in autophagy, breached cell wall integrity, and higher sensitivity to both calcium and heavy metal stress. Transformants constitutively expressing an active MoYPT7 allele (MoYPT7‐CA, Gln67Leu) exhibited distinct phenotypes from the ΔMoYPT7 mutant. Expression of MoYPT7‐CA in MoYpt7 reduced pathogenicity and produced more appressoria‐forming single‐septum conidia. These results indicate that MoYPT7 is required for fungal morphogenesis, vacuole fusion, autophagy, stress resistance and pathogenicity in M. oryzae.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.12903</identifier><identifier>PMID: 25991510</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Autophagy ; Autophagy - genetics ; Calcium - pharmacology ; Cell Wall - metabolism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Hordeum - microbiology ; Hordeum vulgare ; Magnaporthe - genetics ; Magnaporthe - pathogenicity ; Membrane Fusion - genetics ; Membrane Fusion - physiology ; Metals, Heavy - pharmacology ; Molecular Sequence Data ; Oryza - microbiology ; Phenotype ; Plant Diseases - microbiology ; rab GTP-Binding Proteins - genetics ; rab GTP-Binding Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Spores, Fungal - genetics ; Spores, Fungal - metabolism ; Stress, Physiological - genetics ; Vacuoles - metabolism ; Virulence - genetics</subject><ispartof>Environmental microbiology, 2015-11, Vol.17 (11), p.4495-4510</ispartof><rights>2015 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><rights>2015 Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25991510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiao-Hong</creatorcontrib><creatorcontrib>Chen, Si-Miao</creatorcontrib><creatorcontrib>Gao, Hui-Min</creatorcontrib><creatorcontrib>Ning, Guo-Ao</creatorcontrib><creatorcontrib>Shi, Huan-Bin</creatorcontrib><creatorcontrib>Wang, Yao</creatorcontrib><creatorcontrib>Dong, Bo</creatorcontrib><creatorcontrib>Qi, Yao-Yao</creatorcontrib><creatorcontrib>Zhang, Dong-Mei</creatorcontrib><creatorcontrib>Lu, Guo-Dong</creatorcontrib><creatorcontrib>Wang, Zong-Hua</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Lin, Fu-Cheng</creatorcontrib><title>The small GTPase MoYpt7 is required for membrane fusion in autophagy and pathogenicity of Magnaporthe oryzae</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
Rab GTPases are required for vesicle–vacuolar fusion during vacuolar biogenesis in fungi. To date, little is known about the biological functions of the Rab small GTPase components in Magnaporthe oryzae. In this study, we investigated MoYpt7 of M. oryzae, a homologue of the small Ras‐like GTPase Ypt7 in Saccharomyces cerevisiae. Cellular localization assays showed that MoYpt7 was predominantly localized to vacuolar membranes. Using a targeted gene disruption strategy, a ΔMoYPT7 mutant was generated that exhibited defects in mycelial growth and production of conidia. The conidia of the ΔMoYPT7 mutant were malformed and defective in the formation of appressoria. Consequently, the ΔMoYPT7 mutant failed to cause disease in rice and barley. Furthermore, the ΔMoYPT7 mutant showed impairment in autophagy, breached cell wall integrity, and higher sensitivity to both calcium and heavy metal stress. Transformants constitutively expressing an active MoYPT7 allele (MoYPT7‐CA, Gln67Leu) exhibited distinct phenotypes from the ΔMoYPT7 mutant. Expression of MoYPT7‐CA in MoYpt7 reduced pathogenicity and produced more appressoria‐forming single‐septum conidia. These results indicate that MoYPT7 is required for fungal morphogenesis, vacuole fusion, autophagy, stress resistance and pathogenicity in M. oryzae.</description><subject>Amino Acid Sequence</subject><subject>Autophagy</subject><subject>Autophagy - genetics</subject><subject>Calcium - pharmacology</subject><subject>Cell Wall - metabolism</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Magnaporthe - genetics</subject><subject>Magnaporthe - pathogenicity</subject><subject>Membrane Fusion - genetics</subject><subject>Membrane Fusion - physiology</subject><subject>Metals, Heavy - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Oryza - microbiology</subject><subject>Phenotype</subject><subject>Plant Diseases - microbiology</subject><subject>rab GTP-Binding Proteins - genetics</subject><subject>rab GTP-Binding Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - metabolism</subject><subject>Stress, Physiological - genetics</subject><subject>Vacuoles - metabolism</subject><subject>Virulence - genetics</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkc1v0zAYxi0E2ka3MzdkicsuAX_Fbo7QbWXSCpMomuBiOfGb1iOJMzsRhL8edx09cMIXv7Z_j9_HfhB6RclbmsY7KiTLWMHSkhWEP0Mnh53nh5qyY_QyxntCqOKKHKFjlhcFzSk5Qc16Czi2pmnwcn1rIuCV_9YPCruIAzyMLoDFtQ-4hbYMpgNcj9H5DrsOm3Hw_dZsJmw6i3szbP0GOle5YcK-xiuz6Uzvw5A6-DD9NnCKXtSmiXD2NM_Q16vL9eJjdvN5eb14f5M5wSTPrCCmsvWcVkIqWVOoiBElKZSBkvHk285Lbm1lGIh5bqscCssqI0srGcm55DN0vr-3D_5hhDjo1sUKmib592PUVOVcKE6K_0GFkJIrqRL65h_03o-hSw9JFJ8XRJLUfYZeP1Fj2YLVfXCtCZP---UJyPfAT9fAdDinRO8S1bvM9C4__ZiovlxdPxZJl-11Lg7w66Az4YdO5lSu7z4t9cWHxZer73cXOud_AIwRoQ0</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Liu, Xiao-Hong</creator><creator>Chen, Si-Miao</creator><creator>Gao, Hui-Min</creator><creator>Ning, Guo-Ao</creator><creator>Shi, Huan-Bin</creator><creator>Wang, Yao</creator><creator>Dong, Bo</creator><creator>Qi, Yao-Yao</creator><creator>Zhang, Dong-Mei</creator><creator>Lu, Guo-Dong</creator><creator>Wang, Zong-Hua</creator><creator>Zhou, Jie</creator><creator>Lin, Fu-Cheng</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7U9</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>The small GTPase MoYpt7 is required for membrane fusion in autophagy and pathogenicity of Magnaporthe oryzae</title><author>Liu, Xiao-Hong ; Chen, Si-Miao ; Gao, Hui-Min ; Ning, Guo-Ao ; Shi, Huan-Bin ; Wang, Yao ; Dong, Bo ; Qi, Yao-Yao ; Zhang, Dong-Mei ; Lu, Guo-Dong ; Wang, Zong-Hua ; Zhou, Jie ; Lin, Fu-Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4263-d40acdf81c4676f1ec0a4b097aeb23151d8b3ddca2e485dc5e9d2ca6bd6205363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Autophagy</topic><topic>Autophagy - genetics</topic><topic>Calcium - pharmacology</topic><topic>Cell Wall - metabolism</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Hordeum - microbiology</topic><topic>Hordeum vulgare</topic><topic>Magnaporthe - genetics</topic><topic>Magnaporthe - pathogenicity</topic><topic>Membrane Fusion - genetics</topic><topic>Membrane Fusion - physiology</topic><topic>Metals, Heavy - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Oryza - microbiology</topic><topic>Phenotype</topic><topic>Plant Diseases - microbiology</topic><topic>rab GTP-Binding Proteins - genetics</topic><topic>rab GTP-Binding Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - metabolism</topic><topic>Stress, Physiological - genetics</topic><topic>Vacuoles - metabolism</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiao-Hong</creatorcontrib><creatorcontrib>Chen, Si-Miao</creatorcontrib><creatorcontrib>Gao, Hui-Min</creatorcontrib><creatorcontrib>Ning, Guo-Ao</creatorcontrib><creatorcontrib>Shi, Huan-Bin</creatorcontrib><creatorcontrib>Wang, Yao</creatorcontrib><creatorcontrib>Dong, Bo</creatorcontrib><creatorcontrib>Qi, Yao-Yao</creatorcontrib><creatorcontrib>Zhang, Dong-Mei</creatorcontrib><creatorcontrib>Lu, Guo-Dong</creatorcontrib><creatorcontrib>Wang, Zong-Hua</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Lin, Fu-Cheng</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiao-Hong</au><au>Chen, Si-Miao</au><au>Gao, Hui-Min</au><au>Ning, Guo-Ao</au><au>Shi, Huan-Bin</au><au>Wang, Yao</au><au>Dong, Bo</au><au>Qi, Yao-Yao</au><au>Zhang, Dong-Mei</au><au>Lu, Guo-Dong</au><au>Wang, Zong-Hua</au><au>Zhou, Jie</au><au>Lin, Fu-Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The small GTPase MoYpt7 is required for membrane fusion in autophagy and pathogenicity of Magnaporthe oryzae</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2015-11</date><risdate>2015</risdate><volume>17</volume><issue>11</issue><spage>4495</spage><epage>4510</epage><pages>4495-4510</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
Rab GTPases are required for vesicle–vacuolar fusion during vacuolar biogenesis in fungi. To date, little is known about the biological functions of the Rab small GTPase components in Magnaporthe oryzae. In this study, we investigated MoYpt7 of M. oryzae, a homologue of the small Ras‐like GTPase Ypt7 in Saccharomyces cerevisiae. Cellular localization assays showed that MoYpt7 was predominantly localized to vacuolar membranes. Using a targeted gene disruption strategy, a ΔMoYPT7 mutant was generated that exhibited defects in mycelial growth and production of conidia. The conidia of the ΔMoYPT7 mutant were malformed and defective in the formation of appressoria. Consequently, the ΔMoYPT7 mutant failed to cause disease in rice and barley. Furthermore, the ΔMoYPT7 mutant showed impairment in autophagy, breached cell wall integrity, and higher sensitivity to both calcium and heavy metal stress. Transformants constitutively expressing an active MoYPT7 allele (MoYPT7‐CA, Gln67Leu) exhibited distinct phenotypes from the ΔMoYPT7 mutant. Expression of MoYPT7‐CA in MoYpt7 reduced pathogenicity and produced more appressoria‐forming single‐septum conidia. These results indicate that MoYPT7 is required for fungal morphogenesis, vacuole fusion, autophagy, stress resistance and pathogenicity in M. oryzae.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>25991510</pmid><doi>10.1111/1462-2920.12903</doi><tpages>16</tpages></addata></record> |
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subjects | Amino Acid Sequence Autophagy Autophagy - genetics Calcium - pharmacology Cell Wall - metabolism Fungal Proteins - genetics Fungal Proteins - metabolism Hordeum - microbiology Hordeum vulgare Magnaporthe - genetics Magnaporthe - pathogenicity Membrane Fusion - genetics Membrane Fusion - physiology Metals, Heavy - pharmacology Molecular Sequence Data Oryza - microbiology Phenotype Plant Diseases - microbiology rab GTP-Binding Proteins - genetics rab GTP-Binding Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Spores, Fungal - genetics Spores, Fungal - metabolism Stress, Physiological - genetics Vacuoles - metabolism Virulence - genetics |
title | The small GTPase MoYpt7 is required for membrane fusion in autophagy and pathogenicity of Magnaporthe oryzae |
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