Gene Expression in Bovine Oocytes and Cumulus Cells After Meiotic Inhibition with the Cyclin‐Dependent Kinase Inhibitor Butyrolactone I

The aim of this study was to determine the effect of temporary inhibition of meiosis using the cyclin‐dependent kinase inhibitor butyrolactone I (BLI) on gene expression in bovine oocytes and cumulus cells. Immature bovine cumulus–oocyte complexes (COCs) were assigned to groups: (i) Control COCs col...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-08, Vol.47 (4), p.615-624
Main Authors: Leal, CLV, Mamo, S, Fair, T, Lonergan, P
Format: Article
Language:English
Subjects:
4-Butyrolactone - analogs & derivatives
4-Butyrolactone - pharmacology
animal ovaries
Animal reproduction
Animals
Biological and medical sciences
Cattle
Cattle - metabolism
cell cycle
Cell division
Cumulus Cells - cytology
Cumulus Cells - metabolism
Cyclin-Dependent Kinases - antagonists & inhibitors
Embryos
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression - drug effects
Gene Expression Profiling - veterinary
gene expression regulation
genes
In Vitro Oocyte Maturation Techniques - veterinary
Mammalian reproduction. General aspects
Meiosis - drug effects
messenger RNA
oocytes
Oocytes - cytology
Oocytes - metabolism
Protein Kinase Inhibitors - pharmacology
quantitative polymerase chain reaction
Real-Time Polymerase Chain Reaction - veterinary
RNA, Messenger - analysis
Vertebrates: reproduction
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Summary:The aim of this study was to determine the effect of temporary inhibition of meiosis using the cyclin‐dependent kinase inhibitor butyrolactone I (BLI) on gene expression in bovine oocytes and cumulus cells. Immature bovine cumulus–oocyte complexes (COCs) were assigned to groups: (i) Control COCs collected immediately after recovery from the ovary or (ii) after in vitro maturation (IVM) for 24 h, (iii) Inhibited COCs collected 24 h after incubation with 100 μm BLI or (iv) after meiotic inhibition for 24 h followed by IVM for a further 22 h. For mRNA relative abundance analysis, pools of 10 denuded oocytes and respective cumulus cells were collected. Transcripts related to cell cycle regulation and oocyte competence were evaluated in oocytes and cumulus cells by quantitative real‐time PCR (qPCR). Most of the examined transcripts were downregulated (p  0.05) after IVM in control oocytes; only INHBA did not show this pattern in inhibited oocytes. Seven genes were upregulated after IVM in control oocytes (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2011.01932.x