Time-Lapse AFM Imaging of DNA Conformational Changes Induced by Daunorubicin

Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. In this work, we show how atomic force micros...

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Bibliographic Details
Published in:Nano letters 2013-11, Vol.13 (11), p.5679-5684
Main Authors: Alonso-Sarduy, Livan, Longo, Giovanni, Dietler, Giovanni, Kasas, Sandor
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biological and medical sciences
Biotechnology
Cancer
Condensed matter: structure, mechanical and thermal properties
Daunorubicin - pharmacology
Deoxyribonucleic acid
DNA - chemistry
DNA - drug effects
DNA - ultrastructure
Drug Design
Electron, ion, and scanning probe microscopy
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Health
Humans
Imaging
Intercalating Agents - pharmacology
Liquids
Methods. Procedures. Technologies
Microscopy, Atomic Force
Nanostructure
Nucleic Acid Conformation - drug effects
Others
Physics
Scanning probe microscopy: scanning tunneling, atomic force, scanning optical, magnetic force, etc
Structure of solids and liquids
crystallography
Synthesis (chemistry)
Various methods and equipments
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Summary:Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. In this work, we show how atomic force microscopy in liquid can characterize, through time-lapse imaging, the dynamical influence of intercalating agents on the supercoiling of DNA, improving our understanding of the drug’s effect.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl403361f