Melatonin improves the reprogramming efficiency of murine-induced pluripotent stem cells using a secondary inducible system

• Published in: Journal of pineal research 2013-08, Vol.55 (1), p.31-39
• Main Authors: Gao, Shuai, Wang, Zhi-Long, Di, Ke-Qian, Chang, Gang, Tao, Li, An, Lei, Wu, Fu-Jia, Xu, Ji-Qiang, Liu, Ying-Wei, Wu, Zhong-Hong, Li, Xiang-Yun, Gao, Shaorong, Tian, Jian-Hui
• Format: Article
• Language: English
• Subjects: Animals; apoptosis; Brain Chemistry; Cells, Cultured; Chimera - genetics; Chimera - metabolism; Female; Fibroblasts; induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - drug effects; Induced Pluripotent Stem Cells - physiology; Male; melatonin; Melatonin - pharmacology; membrane receptor; Mice; Mice, Inbred ICR; Mice, SCID; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Proto-Oncogene Proteins p21(ras) - genetics; Proto-Oncogene Proteins p21(ras) - metabolism; Receptors, Melatonin - genetics; Receptors, Melatonin - metabolism; secondary inducible system; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0742-3098; 1600-079X
• DOI: 10.1111/jpi.12047

This study focused on the effect of melatonin on reprogramming with specific regard to the generation of induced pluripotent stem cells (iPSCs). Here, a secondary inducible system, which is more accurate and suitable for studying the involvement of chemicals in reprogramming efficiency, was used to evaluate the effect of melatonin on mouse iPSC generation. Secondary fibroblasts collected from all‐iPSC mice through tetraploid complementation were cultured in induction medium supplemented with melatonin at different concentrations (0, 10−6, 10−7, 10−8, 10−9, or 10−10 m) or with vitamin C (50 μg/mL) as a positive control. Compared with untreated group (0.22 ± 0.04% efficiency), 10−8 (0.81 ± 0.04%), and 10−9 m (0.83 ± 0.08%) melatonin supplementation significantly improved reprogramming efficiency (P