l-cysteine replaces microaerophilous culture conditions for the in vitro initiation of Theileria equi

Fifty-one blood samples of carrier horses from Theileria equi -endemic localities in South Africa were used for two different methods of in vitro culture initiation of T. equi parasites. Cultures were initiated either in a oxygen-reduced gas mixture or in a 5 % CO 2 -in-air atmosphere in combination...

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Bibliographic Details
Published in:Parasitology research (1987) 2014, Vol.113 (1), p.433-435
Main Authors: Zweygarth, E., Josemans, A. I.
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological research
Biology, Experimental
Biomedical and Life Sciences
Biomedicine
Carrier State
Culture Media - chemistry
Culture Techniques
Cultures and culture media
Cysteine
Cysteine - chemistry
Health aspects
Horse Diseases - parasitology
Horses
Horses - parasitology
Host-parasite relationships
Immunology
Medical Microbiology
Microbiology
Original Paper
Physiological aspects
South Africa
Theileria
Theileria - growth & development
Theileria equi
Theileriasis - parasitology
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Summary:Fifty-one blood samples of carrier horses from Theileria equi -endemic localities in South Africa were used for two different methods of in vitro culture initiation of T. equi parasites. Cultures were initiated either in a oxygen-reduced gas mixture or in a 5 % CO 2 -in-air atmosphere in combination with l -cysteine-supplemented culture medium. Out of the 51 blood samples, 43 and 42 cultures, respectively, became culture positive. A possible explanation for this observation is proposed.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-013-3672-0