Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus)

We determined the effect of enrofloxacin on the lactate dehydrogenase (LDH) release, reactive oxygen species (ROS), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde (MDA), mitochondria membrane potential (ΔΨm) and apoptosis in the hepatic cell line of grass carp (Cteno...

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Bibliographic Details
Published in:Fish & shellfish immunology 2015-12, Vol.47 (2), p.639-644
Main Authors: Liu, Bo, Cui, Yanting, Brown, Paul B., Ge, Xianping, Xie, Jun, Xu, Pao
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - toxicity
Antioxidants - metabolism
Apoptosis
Apoptosis - drug effects
Carps - physiology
Cell Line
Ctenopharyngodon idella
Cytotoxicity
Enrofloxacin
Fluoroquinolones - toxicity
Freshwater
Grass carp
Hepatic cell
Liver
Membrane Potential, Mitochondrial - drug effects
Reactive Oxygen Species - metabolism
ROS
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Summary:We determined the effect of enrofloxacin on the lactate dehydrogenase (LDH) release, reactive oxygen species (ROS), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde (MDA), mitochondria membrane potential (ΔΨm) and apoptosis in the hepatic cell line of grass carp (Ctenopharyngodon idellus). Cultured cells were treated with different concentrations of enrofloxacin (12.5–200 ug/mL) for 24 h. We found that the cytotoxic effect of enrofloxacin was mediated by apoptosis, and that this apoptosis occurred in a dose-dependent manner. The doses of 50,100 and 200 μg/mL enrofloxacin increased the LDH release and MDA concentration, induced cell apoptosis and reduced the ΔΨm compared to the control. The highest dose of 200 ug/mL enrofloxacin also significantly induced apoptosis accompanied by ΔΨm disruption and ROS generation and significantly reduced T-AOC and increased MDA concentration compared to the control. Our results suggest that the dose of 200 ug/mL enrofloxacin exerts its cytotoxic effect and produced ROS via apoptosis by affecting the mitochondria of the hepatic cells of grass carp. •Enrofloxacin has the potential to cytotoxic effect of fish.•Enrofloxacin has the potential to induce cell oxidation stress of fish.•Enrofloxacin has the potential to induce cell apoptosis induction of fish.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2015.10.007