Optical Characterization of the Antigen–Antibody Thin Layer Using the Whispering Gallery Mode

We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e.,...

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Bibliographic Details
Published in:Analytical Sciences 2014/08/10, Vol.30(8), pp.799-804
Main Authors: TAJIRI, Takeshi, MATSUMOTO, Shuzo, IMATO, Toshihiko, OKAMOTO, Toshihiro, HARAGUCHI, Masanobu
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antigen-Antibody Reactions
biosensors
Chemistry
Covalent bonds
Mathematical analysis
Microsphere
Microspheres
Optics and Photonics
Original Papers
Refractive index
Refractivity
resonance sensors
Spectra
Thin films
whispering gallery mode
Whispering gallery modes
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Summary:We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen–antibody layer from the shift of the WGM spectra peak.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.30.799