Organisation and expression of mitochondrial atp9 genes from CMS and fertile carrots

• Published in: Theoretical and applied genetics 2000-01, Vol.100 (2), p.263-270
• Main Authors: SZKLARCZYK, M, OCZKOWSKI, M, AUGUSTYNIAK, H, BÖRNER, T, LINKE, B, MICHALIK, B
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; Agronomy. Soil science and plant productions; Biological and medical sciences; Carrots; Classical genetics, quantitative genetics, hybrids; Cloning; Cytoplasm; cytoplasmic male sterility; Daucus carota; Economic plant physiology; Fertility; Flowers & plants; Fructification, ripening. Postharvest physiology; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Genetic aspects; Genetics of eukaryotes. Biological and molecular evolution; Growth and development; Life sciences; Mitochondrial DNA; Physiological aspects; Plant genetics; Plant mitochondria; Pteridophyta, spermatophyta; rRNA 5S; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050035

The [F.sub.0]-[F.sub.1] ATPase subunit 9 gene of carrot mitochondria has been isolated from both petaloid malesterile (atp9-1) and normal fertile cytoplasms (atp9-3). The position occupied in atp9-3 by the TAA stop codon is, in the case of atp9-l replaced by the CAA triplet coding for glutamine, which makes this latter ORF 13 amino acid residues longer than that of the atp9-3. The 3' end of atp9-3 gene is flanked by a direct repeat of 42 bp. The sequence of the repeat unit is also present at the 3' end of atp9-1 but without reiteration. A truncated and presumably inactive version of atp9 (atp9-2) was found to be present in cytoplasms regardless of the fertility phenotype which they condition. The atp9-l gene from petaloid cytoplasm appeared to be co-transcribed with the gene coding for 5S rRNA, and nuclear background influenced the accumulation of the respective transcript. The results are discussed with respect to a potential role of atp9-1 in generating the petaloid form of CMS.