Dansyl-Labeled Cholic Acid as a Tool To Build Speciation Diagrams for the Aggregation of Bile Acids

Bile acids (BAs) are a family of natural steroids biosynthesized from cholesterol in the liver that tend to form aggregates in solution. A fluorescent derivative of cholic acid, namely 3α-Dns-ChA, was employed as a reporter to establish the speciation diagrams of the most abundant BAs that can be fo...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2012-12, Vol.116 (51), p.14776-14780
Main Authors: Gomez-Mendoza, Miguel, Marin, M. Luisa, Miranda, Miguel A
Format: Article
Language:English
Subjects:
Agglomeration
Aggregates
Bile Acids and Salts - chemistry
Cholic Acid - chemistry
Construction equipment
Derivatives
Phosphatidylcholines - chemistry
Quantum Theory
Sodium
Speciation
Spectrometry, Fluorescence
Steroids
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Summary:Bile acids (BAs) are a family of natural steroids biosynthesized from cholesterol in the liver that tend to form aggregates in solution. A fluorescent derivative of cholic acid, namely 3α-Dns-ChA, was employed as a reporter to establish the speciation diagrams of the most abundant BAs that can be found mainly in three microenvironments, solution, and primary and secondary aggregates. The developed methodology is based on the analysis of the combined steady-state and time-resolved experiments performed on 3α-Dns-ChA whose emission behavior was found to be strongly medium dependent. In particular, speciation diagrams of sodium glycocholate (NaGCh), sodium taurocholate (NaTCh), sodium chenodeoxycholate (NaCDCh), sodium glycochenodeoxycholate (NaGCDCh), sodium deoxycholate (NaDCh), and sodium ursodeoxycholate (NaUDCh) were successfully built up.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp308624h