Ethanol-Induced Perturbations to Planar Lipid Bilayer Structures

We report on the formation of planar lipid bilayer structures on mica where the bilayer contains the phosphocholine 1,2-dioleoyl-sn-phosphatidylcholine (DOPC), cholesterol, sphingomyelin and sulforhodamine-tagged-1,2-dioleoyl-sn-phosphatidylethanolamine (SR-DOPE). Phase separation is seen for the ch...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2014-01, Vol.118 (2), p.537-546
Main Authors: Setiawan, Iwan, Blanchard, G. J
Format: Article
Language:English
Subjects:
Cholesterol
Cholesterol - chemistry
Ethanol
Ethanol - chemistry
Ethyl alcohol
Fluorescence
Fluorescence Polarization
Hydrogen Bonding
Imaging
Lipid Bilayers - chemistry
Lipids
Mica
Phosphatidylcholines - chemistry
Phosphatidylethanolamines - chemistry
Sphingomyelins - chemistry
Steady state
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Summary:We report on the formation of planar lipid bilayer structures on mica where the bilayer contains the phosphocholine 1,2-dioleoyl-sn-phosphatidylcholine (DOPC), cholesterol, sphingomyelin and sulforhodamine-tagged-1,2-dioleoyl-sn-phosphatidylethanolamine (SR-DOPE). Phase separation is seen for the cholesterol domains within the bilayer structure, and exposure of this supported bilayer to controlled concentrations of ethanol reveals organizational changes on both the micrometer- and molecular-length scales. We report steady state fluorescence imaging, fluorescence lifetime imaging, and fluorescence anisotropy decay imaging for these bilayers. These data are complementary to existing information on the interactions of lipid bilayers with ethanol and point to subtle but important changes in the molecular-scale organization of these structures.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp410305m