Phosphatidylcholine nanovesicles coated with chitosan or chondroitin sulfate as novel devices for bacteriocin delivery

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2014-07, Vol.16 (7), p.1-10, Article 2479
• Main Authors: da Silva, Indjara Mallmann, Boelter, Juliana Ferreira, da Silveira, Nádya Pesce, Brandelli, Adriano
• Format: Article
• Language: English
• Subjects: Antiinfectives and antibacterials; Bacteria; Biological and medical sciences; Biotechnology; Characterization and Evaluation of Materials; Chemistry and Materials Science; Chitosan; Computing time; Encapsulation; Food preservation; Fundamental and applied biological sciences. Psychology; Inorganic Chemistry; Lasers; Listeria; Listeria monocytogenes; Materials Science; Methods. Procedures. Technologies; Nanoparticles; Nanostructure; Nanotechnology; Nisin; Optical Devices; Optics; Others; Peptides; Photonics; Physical Chemistry; Research Paper; Saccharides; Sulfates; Thin films; Various methods and equipments; Zeta potential
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-014-2479-y

There is increased interest on the use of natural antimicrobial peptides in biomedicine and food preservation technologies. In this study, the antimicrobial activity of nisin encapsulated into nanovesicles containing polyanionic polysaccharides was investigated. Nisin was encapsulated in phosphatidylcholine (PC) liposomes containing chitosan or chondroitin sulfate by the thin-film hydration method and tested for antimicrobial activity against Listeria spp. The mean particle size of PC liposomes was 145 nm and varied to 210 and 134 nm with the incorporation of chitosan and chondroitin sulfate, respectively. Nisin-containing nanovesicles with and without incorporation of polysaccharides had a zeta potential values around −20 mV, showing mostly spherical structures when observed by transmission electron microscopy. Encapsulated nisin had similar efficiency as free nisin in inhibiting Listeria spp. isolated from bovine carcass, and greater efficiency in inhibiting Listeria monocytogenes . The formulation containing chitosan was more stable and more efficient in inhibiting L. monocytogenes when compared to the other nanovesicles tested. After 24 h, the viable cell counts were 2 log lower as compared with the other treatments and 7 log comparing to controls.