Isolation of five polymorphic microsatellite loci in the invasive weed Ambrosia artemisiifolia (Asteraceae) using an enrichment protocol

We report the development of five microsatellite markers for the invasive North American native plant Ambrosia artemisiifolia (Asteraceae). An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with samples from 16 natural populations collected in the native ra...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.381-383
Main Authors: Genton, B.J, Jonot, O, Thevenet, D, Fournier, E, Blatrix, R, Vautrin, D, Solignac, M, Giraud, T
Format: Article
Language:English
Subjects:
alleles
alleles per locus
Ambrosia artemisiifolia
bioinvasion
DNA primers
enriched library
exotic
genetic markers
heterozygosity
introduction
invasive species
loci
microsatellite repeats
molecular sequence data
nucleotide sequences
polymerase chain reaction
polymorphic loci
ragweed
weeds
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Summary:We report the development of five microsatellite markers for the invasive North American native plant Ambrosia artemisiifolia (Asteraceae). An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with samples from 16 natural populations collected in the native range, across North America, and in an invaded area along the Rhône Valley in France. In these areas, we found a high level of polymorphism within population and significant heterozygote deficiencies.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00934.x