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Isolation of five polymorphic microsatellite loci in the invasive weed Ambrosia artemisiifolia (Asteraceae) using an enrichment protocol
We report the development of five microsatellite markers for the invasive North American native plant Ambrosia artemisiifolia (Asteraceae). An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with samples from 16 natural populations collected in the native ra...
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Published in: | Molecular ecology notes 2005-06, Vol.5 (2), p.381-383 |
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container_issue | 2 |
container_start_page | 381 |
container_title | Molecular ecology notes |
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creator | Genton, B.J Jonot, O Thevenet, D Fournier, E Blatrix, R Vautrin, D Solignac, M Giraud, T |
description | We report the development of five microsatellite markers for the invasive North American native plant Ambrosia artemisiifolia (Asteraceae). An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with samples from 16 natural populations collected in the native range, across North America, and in an invaded area along the Rhône Valley in France. In these areas, we found a high level of polymorphism within population and significant heterozygote deficiencies. |
doi_str_mv | 10.1111/j.1471-8286.2005.00934.x |
format | article |
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language | eng |
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source | Wiley |
subjects | alleles alleles per locus Ambrosia artemisiifolia bioinvasion DNA primers enriched library exotic genetic markers heterozygosity introduction invasive species loci microsatellite repeats molecular sequence data nucleotide sequences polymerase chain reaction polymorphic loci ragweed weeds |
title | Isolation of five polymorphic microsatellite loci in the invasive weed Ambrosia artemisiifolia (Asteraceae) using an enrichment protocol |
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