Micropropagation of zedoary (Curcuma zedoaria Roscoe)--a valuable medicinal plant

Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l super(-1) sucrose and 5 g l super(-1) agar, supplemented with 20 (v/v) coconut wate...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2005-04, Vol.81 (1), p.119-122
Main Authors: Nguyen Hoang Loc, Doan Trong Duc, Kwon, T.H, Yang, M.S
Format: Article
Language:English
Subjects:
benzyladenine
Biological and medical sciences
Biotechnology
charcoal
coconut milk
culture media
Curcuma
Curcuma zedoaria
dose response
Eukaryotic cell cultures
explants
Fundamental and applied biological sciences. Psychology
in vitro culture
in vitro regeneration
indole butyric acid
kinetin
medicinal plants
methodology
Methods. Procedures. Technologies
micropropagation
Miscellaneous
naphthaleneacetic acid
Plant cells and fungal cells
rhizomes
shoots
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Summary:Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l super(-1) sucrose and 5 g l super(-1) agar, supplemented with 20 (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l super(-1). The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20 (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20 (v/v) CW, 3 mg l super(-1) BA, and 0.5 mg l super(-1) IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30-40 mm in length) were rooted on MS medium containing 20 g l super(-1) sucrose and 8 g l super(-1) agar, supplemented with 20 (v/v) CW and 2 mg l super(-1) NAA. More than 95 of the rooted plants were established in pots after hardening.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-004-3308-2