Snowdrop lectin (GNA) has no acute toxic effects on a beneficial insect predator, the 2-spot ladybird ( Adalia bipunctata L.)

• Published in: Journal of insect physiology 2000-04, Vol.46 (4), p.379-391
• Main Authors: Down, Rachel E., Ford, Louise, Woodhouse, Stephen D., Raemaekers, Romaan J.M., Leitch, Beulah, Gatehouse, John A., Gatehouse, Angharad M.R.
• Format: Article
• Language: English
• Subjects: Adalia bipunctata; adverse effects; agglutinins; Aphididae; beneficial insects; binding; biological development; Coccinellidae; Crop protection; food intake; Galanthus nivalis; galanthus nivalis agglutinin; Galanthus nivalis agglutinin (GNA); interactions; Ladybird larvae; larvae; lectin; Lectin binding; lectins; microvilli; midgut; mortality; Myzus persicae; nontarget effects; nontarget organisms; predators; predators of insect pests; tritrophic interactions; Tritrophic level
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/S0022-1910(99)00121-3

Two-spot ladybird ( Adalia bipunctata L.) larvae were fed on aphids ( Myzus persicae (Sulz.)) which had been loaded with snowdrop lectin ( Galanthus nivalis agglutinin; GNA) by feeding on artificial diet containing the protein. Treatment with GNA significantly decreased the growth of aphids. No acute toxicity of GNA-containing aphids towards the ladybird larvae was observed, although there were small effects on development. When fed a fixed number of aphids, larvae exposed to GNA spent longer in the 4th instar, taking 6 extra days to reach pupation; however, retardation of development was not observed in ladybird larvae fed equal weights of aphids. Ladybird larvae fed GNA-containing aphids were found to be 8–15% smaller than controls, but ate a significantly greater number of aphids (approx. 40% to pupation). GNA was shown to be present on the microvilli of the midgut brush border membrane and within gut epithelial cells in ladybird larvae fed on GNA-dosed aphids, although disruption of the brush border was not observed. It is hypothesised that GNA does not have significant direct toxic or adverse effects on developing ladybird larvae, but that the effects observed may be due to the fact that the aphids fed on GNA are compromised and are thus a suboptimal food.