Multistress Regulation in Escherichia coli: Expression of osmB Involves Two Independent Promoters Responding either to sigma super(S) or to the RcsCDB His-Asp Phosphorelay

Transcription of the Escherichia coli osmB gene is induced by several stress conditions. osmB is expressed from two promoters, osmBp1 and osmBp2. The downstream promoter, osmBp2, is induced after osmotic shock or upon entry into stationary phase in a sigma super(S)-dependent manner. The upstream pro...

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Bibliographic Details
Published in:Journal of bacteriology 2005-05, Vol.187 (9), p.3282-3286
Main Authors: Boulanger, Alice, Francez-Charlot, Anne, Conter, Annie, Castanie-Cornet, Marie-Pierre, Cam, Kaymeuang, Gutierrez, Claude
Format: Article
Language:English
Subjects:
Escherichia coli
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Summary:Transcription of the Escherichia coli osmB gene is induced by several stress conditions. osmB is expressed from two promoters, osmBp1 and osmBp2. The downstream promoter, osmBp2, is induced after osmotic shock or upon entry into stationary phase in a sigma super(S)-dependent manner. The upstream promoter, osmBp1, is independent of sigma super(S) and is activated by RcsB, the response regulator of the His-Asp phosphorelay signal transduction system RcsCDB. RcsB is responsible for the induction of osmBp1 following treatment with chlorpromazine. Activation of osmBp1 by RcsB requires a sequence upstream of its -35 element similar to the RcsB binding site consensus, suggesting a direct regulatory role. osmB appears as another example of a multistress-responsive gene whose transcription involves both a sigma super(S)-dependent promoter and a second one independent of sigma super(S) but controlled by stress-specific transcription factors.
ISSN:0021-9193