Development and characterization of microsatellite markers from the yellow passion fruit (Passiflora edulis f. flavicarpa)

Here we described the development of the first set of Passiflora microsatellite loci isolated from an enriched genomic library. A sample of 43 individuals from 12 accessions of the yellow passion fruit was used to characterize those loci, which revealed up to 20 alleles per locus. Two loci were mono...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.331-333
Main Authors: Oliveira, E.J, Padua, J.G, Zucchi, M.I, Camargo, L.E.A, Fungaro, M.H.P, Vieira, M.L.C
Format: Article
Language:English
Subjects:
alleles
alleles per locus
DNA primers
genetic diversity
genetic markers
genetic variation
heterozygosity
loci
microsatellite repeats
molecular markers
molecular sequence data
nucleotide sequences
Passiflora edulis
Passiflora edulis f. flavicarpa
passion fruit
passion fruits
polymerase chain reaction
polymorphic loci
SSR
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Summary:Here we described the development of the first set of Passiflora microsatellite loci isolated from an enriched genomic library. A sample of 43 individuals from 12 accessions of the yellow passion fruit was used to characterize those loci, which revealed up to 20 alleles per locus. Two loci were monomorphic. The observed (HO) and expected (HE) heterozygosities were very similar, as expected for a self‐incompatible species. Allelic diversity (HT) was 0.444. This set of markers will permit genetic structure analyses of cultivated and wild populations of Passiflora, and contribute for integrating genetic maps based on dominant markers, as they can provide bridge alleles.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00917.x