Isolation and characterization of six polymorphic microsatellite loci in Aspergillus niger

Certain strains of Aspergillus niger produce ochratoxin A in food and in animal feeds. Six polymorphic microsatellite markers suitable for population analysis were developed for A. niger through screening published sequences for microsatellite repeats. Polymorphism was evaluated for 28 isolates of A...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.375-377
Main Authors: Esteban, A, Leong, S.L.L, Tran-Dinh, N
Format: Article
Language:English
Subjects:
alleles
alleles per locus
Aspergillus carbonarius
Aspergillus japonicus
Aspergillus niger
cross-species amplification
DNA primers
genetic markers
heterozygosity
loci
microsatellite repeats
microsatellites
molecular sequence data
nucleotide sequences
ochratoxin A
polymerase chain reaction
polymorphic loci
toxigenic strains
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Summary:Certain strains of Aspergillus niger produce ochratoxin A in food and in animal feeds. Six polymorphic microsatellite markers suitable for population analysis were developed for A. niger through screening published sequences for microsatellite repeats. Polymorphism was evaluated for 28 isolates of A. niger, including toxigenic strains. Loci displayed six to 13 alleles. Investigation of cross‐species amplifications with Aspergillus carbonarius and Aspergillus japonicus showed limited success.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00932.x