Isolation and characterization of 10 microsatellite loci from Iris hexagona (Iridaceae)

Ten microsatellite loci isolated from Iris hexagona are described. All these loci are polymorphic, with three to 13 alleles across 24 individuals from a single natural population. Heterozygosity ranged from 0.125 to 0.870. Three loci depart significantly from Hardy–Weinberg equilibrium in our test p...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.410-412
Main Authors: Meerow, A.W, Gideon, M, Kuhn, D.N, Schnell, R.J
Format: Article
Language:English
Subjects:
alleles
alleles per locus
DNA primers
genetic markers
heterozygosity
Iris
Iris (Iridaceae)
Iris hexagona
linkage disequilibrium
loci
Louisiana iris
microsatellite
microsatellite repeats
molecular sequence data
nucleotide sequences
polymerase chain reaction
polymorphic loci
population genetics
SSR
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Summary:Ten microsatellite loci isolated from Iris hexagona are described. All these loci are polymorphic, with three to 13 alleles across 24 individuals from a single natural population. Heterozygosity ranged from 0.125 to 0.870. Three loci depart significantly from Hardy–Weinberg equilibrium in our test population. The test population shows significant heterozygote deficiency in these and two other loci. Three loci exhibit significant linkage disequilibrium. These loci will be utilized to investigate patterns of genetic variation in the species throughout the Florida peninsula.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00943.x