Doxycycline, the drug used to control the tet‐regulatable promoter system, has no effect on global gene expression in Saccharomyces cerevisiae

The tet‐regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2005-05, Vol.22 (7), p.565-569
Main Authors: Wishart, Jill A., Hayes, Andrew, Wardleworth, Leanne, Zhang, Nianshu, Oliver, Stephen G.
Format: Article
Language:English
Subjects:
Affymetrix arrays
Culture Media
doxycycline
Doxycycline - pharmacology
essential genes
Gene Expression Profiling
Gene Expression Regulation, Fungal - drug effects
global gene expression
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic
Proteome
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Tetracycline - pharmacology
tetracycline‐regulatable promoter
Transcription, Genetic
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Summary:The tet‐regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is widely accepted that doxycycline is innocuous to yeast. Global genetic studies are now commonplace and the tetO‐system is being used in transcriptome studies. Hence, we wanted to ensure that the absence of phenotypic effects, on addition of doxycycline to the growth medium, is mirrored in transcriptome data. We have demonstrated that doxycycline has no significant effect on global transcription levels and will continue to use the tetO‐regulatable promoter system for genetic studies. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1225