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Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales, Cyanobacteria)
In populations of Planktothrix, microcystin-producers and non-producers, which are morphologically identical, coexist. In order to develop a basis for the reliable detection of microcystin producers in field samples with polymerase chain reaction (PCR) based methods, we studied the presence and vari...
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| Published in: | FEMS microbiology letters 2005-04, Vol.245 (2), p.299-306 |
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| container_end_page | 306 |
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| container_title | FEMS microbiology letters |
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| creator | Mbedi, Susan Welker, Martin Fastner, Jutta Wiedner, Claudia |
| description | In populations of
Planktothrix, microcystin-producers and non-producers, which are morphologically identical, coexist. In order to develop a basis for the reliable detection of microcystin producers in field samples with polymerase chain reaction (PCR) based methods, we studied the presence and variability of eight regions of the
mcy gene cluster in 46
Planktothrix strains, including both microcystin-producing and non-producing ones. PCR-amplification products for two
mcy gene regions were also found in non-microcystin-producing strains, indicating the existence of natural mutants. PCR-products of the other regions studied were only detected in microcystin-producing strains. Two of these
mcy-amplicons were variable in sequence and length. Four gene regions remained that were conserved and specific for microcystin-producing
Planktothrix strains, and thus qualified to detect the respective chemotypes in environmental samples. |
| doi_str_mv | 10.1016/j.femsle.2005.03.020 |
| format | article |
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Planktothrix, microcystin-producers and non-producers, which are morphologically identical, coexist. In order to develop a basis for the reliable detection of microcystin producers in field samples with polymerase chain reaction (PCR) based methods, we studied the presence and variability of eight regions of the
mcy gene cluster in 46
Planktothrix strains, including both microcystin-producing and non-producing ones. PCR-amplification products for two
mcy gene regions were also found in non-microcystin-producing strains, indicating the existence of natural mutants. PCR-products of the other regions studied were only detected in microcystin-producing strains. Two of these
mcy-amplicons were variable in sequence and length. Four gene regions remained that were conserved and specific for microcystin-producing
Planktothrix strains, and thus qualified to detect the respective chemotypes in environmental samples.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2005.03.020</identifier><identifier>PMID: 15837386</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; biosynthesis ; chemotypes ; Conserved sequence ; Cyanobacteria ; Cyanobacteria - enzymology ; Cyanobacteria - genetics ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. Psychology ; Gene Order ; Genes, Bacterial ; Genetic Variation ; Genetics ; mcy-Genes ; Microbiology ; Microcystin ; microcystin-nonproducing strains ; microcystin-producing strains ; Microcystins ; Molecular Sequence Data ; Multigene Family ; myc gene cluster ; Oscillatoriales ; Peptide Synthases - genetics ; Peptides, Cyclic - biosynthesis ; Planktothrix ; Polymerase Chain Reaction ; Sequence Analysis, DNA</subject><ispartof>FEMS microbiology letters, 2005-04, Vol.245 (2), p.299-306</ispartof><rights>2005 Federation of European Microbiological Societies</rights><rights>2005 Federation of European Microbiological Societies 2005</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5779-e40415ca83dd4c577edf103d4f815db2bfd1cee56be7dc42a6d60a4b510437113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16705027$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15837386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mbedi, Susan</creatorcontrib><creatorcontrib>Welker, Martin</creatorcontrib><creatorcontrib>Fastner, Jutta</creatorcontrib><creatorcontrib>Wiedner, Claudia</creatorcontrib><title>Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales, Cyanobacteria)</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>In populations of
Planktothrix, microcystin-producers and non-producers, which are morphologically identical, coexist. In order to develop a basis for the reliable detection of microcystin producers in field samples with polymerase chain reaction (PCR) based methods, we studied the presence and variability of eight regions of the
mcy gene cluster in 46
Planktothrix strains, including both microcystin-producing and non-producing ones. PCR-amplification products for two
mcy gene regions were also found in non-microcystin-producing strains, indicating the existence of natural mutants. PCR-products of the other regions studied were only detected in microcystin-producing strains. Two of these
mcy-amplicons were variable in sequence and length. Four gene regions remained that were conserved and specific for microcystin-producing
Planktothrix strains, and thus qualified to detect the respective chemotypes in environmental samples.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>chemotypes</subject><subject>Conserved sequence</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - enzymology</subject><subject>Cyanobacteria - genetics</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Order</subject><subject>Genes, Bacterial</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>mcy-Genes</subject><subject>Microbiology</subject><subject>Microcystin</subject><subject>microcystin-nonproducing strains</subject><subject>microcystin-producing strains</subject><subject>Microcystins</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>myc gene cluster</subject><subject>Oscillatoriales</subject><subject>Peptide Synthases - genetics</subject><subject>Peptides, Cyclic - biosynthesis</subject><subject>Planktothrix</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkl9rFDEUxQdRbK1-A9GBoii425v_My-CLFaFlQpaX0MmudNmnZ1skxl1vr1ZZ6Hgg_QpcPI79x5yUhRPCSwJEHm2Wba4TR0uKYBYAlsChXvFMRGKL2Qtq_vFMTBVLQjU6qh4lNIGADgF-bA4IqJiilXyuLj5bqI3je_8MJWhLYdrLLfexmCnNPi-TFOfpcEkLK-wx9J2Yxowlvlqj2ZtTOWXzvQ_hjBcR_-7fHWRrO86M4Q8uMP0plxNpg-NsdnnzevHxYPWdAmfHM6T4vL8_bfVx8X64sOn1bv1wgql6gVy4ERYUzHn-F5C1xJgjrcVEa6hTeuIRRSyQeUsp0Y6CYY3ggBnihB2Uryc5-5iuBkxDXrrk8WcrMcwJk2U4AB_wdN_wE0YY5-zacpACkkJ5ZniM5XfJqWIrd5FvzVx0gT0vhC90XMhel-IBqZzIdn27DB8bLbobk2HBjLw4gCYZE3XRtNbn245qUAAVZmrZu6X73C603J9_nlN6zpbz2ZrGHd3Tf18drQmaHMVc6DLrxQIA6jr_IlEJt7OBOYKf3qMOreOvUXnI9pBu-D_v-IPX0rWSw</recordid><startdate>20050415</startdate><enddate>20050415</enddate><creator>Mbedi, Susan</creator><creator>Welker, Martin</creator><creator>Fastner, Jutta</creator><creator>Wiedner, Claudia</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20050415</creationdate><title>Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales, Cyanobacteria)</title><author>Mbedi, Susan ; Welker, Martin ; Fastner, Jutta ; Wiedner, Claudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5779-e40415ca83dd4c577edf103d4f815db2bfd1cee56be7dc42a6d60a4b510437113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>chemotypes</topic><topic>Conserved sequence</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - enzymology</topic><topic>Cyanobacteria - genetics</topic><topic>DNA, Bacterial - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Order</topic><topic>Genes, Bacterial</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>mcy-Genes</topic><topic>Microbiology</topic><topic>Microcystin</topic><topic>microcystin-nonproducing strains</topic><topic>microcystin-producing strains</topic><topic>Microcystins</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>myc gene cluster</topic><topic>Oscillatoriales</topic><topic>Peptide Synthases - genetics</topic><topic>Peptides, Cyclic - biosynthesis</topic><topic>Planktothrix</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mbedi, Susan</creatorcontrib><creatorcontrib>Welker, Martin</creatorcontrib><creatorcontrib>Fastner, Jutta</creatorcontrib><creatorcontrib>Wiedner, Claudia</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mbedi, Susan</au><au>Welker, Martin</au><au>Fastner, Jutta</au><au>Wiedner, Claudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales, Cyanobacteria)</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2005-04-15</date><risdate>2005</risdate><volume>245</volume><issue>2</issue><spage>299</spage><epage>306</epage><pages>299-306</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>In populations of
Planktothrix, microcystin-producers and non-producers, which are morphologically identical, coexist. In order to develop a basis for the reliable detection of microcystin producers in field samples with polymerase chain reaction (PCR) based methods, we studied the presence and variability of eight regions of the
mcy gene cluster in 46
Planktothrix strains, including both microcystin-producing and non-producing ones. PCR-amplification products for two
mcy gene regions were also found in non-microcystin-producing strains, indicating the existence of natural mutants. PCR-products of the other regions studied were only detected in microcystin-producing strains. Two of these
mcy-amplicons were variable in sequence and length. Four gene regions remained that were conserved and specific for microcystin-producing
Planktothrix strains, and thus qualified to detect the respective chemotypes in environmental samples.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>15837386</pmid><doi>10.1016/j.femsle.2005.03.020</doi><tpages>8</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 2005-04, Vol.245 (2), p.299-306 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Bacterial Proteins - genetics Bacteriology Biological and medical sciences biosynthesis chemotypes Conserved sequence Cyanobacteria Cyanobacteria - enzymology Cyanobacteria - genetics DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology Gene Order Genes, Bacterial Genetic Variation Genetics mcy-Genes Microbiology Microcystin microcystin-nonproducing strains microcystin-producing strains Microcystins Molecular Sequence Data Multigene Family myc gene cluster Oscillatoriales Peptide Synthases - genetics Peptides, Cyclic - biosynthesis Planktothrix Polymerase Chain Reaction Sequence Analysis, DNA |
| title | Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales, Cyanobacteria) |
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