Effects of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2005-04, Vol.81 (1), p.105-108
• Main Authors: Zhou, Y.H, Guo, D.P, Zhu, Z.J, Qian, Q.Q
• Format: Article
• Language: English
• Subjects: acclimation; Biological and medical sciences; Biotechnology; cell growth; Eukaryotic cell cultures; Fragaria ananassa; Fundamental and applied biological sciences. Psychology; in vitro culture; methodology; Methods. Procedures. Technologies; micropropagation; Miscellaneous; photosynthesis; Plant cells and fungal cells; rooting; shoots; solar radiation; strawberries
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-004-2775-9

Strawberry (Fragaria ananassa Duch. cv. Fengxiang) plantlets were cultured under two in vitro environments for rooting, and then acclimatized under two ex vitro irradiance conditions. At the end of rooting stage plant height, fresh weight and specific leaf area of T1-plants grown under high sucrose concentration (3 sucrose), low photosynthetic photon flux density (30 mu mol m super(-2) s super(-1)) and normal CO sub(2) concentration (350-400 mu l l super(-1)) were significantly higher than those of T2-plantlets grown under low sucrose concentration (0.5), high photosynthetic photon flux density (90 mu mol m super(-2) s super(-1)) and elevated CO sub(2) concentration (700-800 mu l l super(-1)). But T2-plantlets had higher net photosynthetic rate (Pn), effective photochemical quantum yield of PSII ( Phi sub(PSII)), effective photosynthetic electron transport rate (ETR), photochemical quenching (q sub()P) and ratio of chlorophyll fluorescence yield decrease (Rfd). After transfer, higher irradiance obviously promoted the growth of plantlets and was beneficial for the development of photosynthetic functions during acclimatization. T2-plantlets had higher fresh weight, leaf area, Phi sub(PSII) and ETR under higher ex vitroirradiance condition.