Skin Damage Mechanisms Related to Airborne Particulate Matter Exposure

Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main orga...

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Bibliographic Details
Published in:Toxicological sciences 2016-01, Vol.149 (1), p.227-236
Main Authors: Magnani, Natalia D, Muresan, Ximena M, Belmonte, Giuseppe, Cervellati, Franco, Sticozzi, Claudia, Pecorelli, Alessandra, Miracco, Clelia, Marchini, Timoteo, Evelson, Pablo, Valacchi, Giuseppe
Format: Article
Language:English
Subjects:
Cells, Cultured
Cytochrome P-450 CYP1A1 - genetics
Humans
Inflammation - complications
Interleukin-1alpha - physiology
L-Lactate Dehydrogenase - secretion
NF-E2-Related Factor 2 - physiology
NF-kappa B - physiology
Oxidative Stress
Particulate Matter - toxicity
Reactive Oxygen Species - metabolism
Skin - drug effects
Skin - ultrastructure
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Summary:Epidemiological studies suggest a correlation between increased airborne particulate matter (PM) and adverse health effects. The mechanisms of PM-health effects are believed to involve oxidative stress and inflammation. To evaluate the ability of PM promoting skin tissue damage, one of the main organs exposed to outdoor pollutants, we analyzed the effect of concentrated ambient particles (CAPs) in a reconstructed human epidermis (RHE) model. RHE tissues were exposed to 25 or 100 µg/ml CAPs for 24 or 48 h. Data showed that RHE seems to be more susceptible to CAPs-induced toxicity after 48 h exposure than after 24 h. We found a local reactive O(2) species (ROS) production increase generated from metals present on the particle, which contributes to lipids oxidation. Furthermore, as a consequence of altered redox status, NFkB nucleus translocation was increase upon CAPs exposure, as well as cyclooxygenase 2 and cytochrome P450 levels, which may be involved in the inflammatory response initiated by PM. CAPs also triggered an apoptotic process in skin. Surprisingly, by transition electron microscopy analysis we showed that CAPs were able to penetrate skin tissues. These findings contribute to the understanding of the cutaneous pathophysiological mechanisms initiated by CAPs exposure, where oxidative stress and inflammation may play predominant roles.
ISSN:1096-6080
1096-0929
DOI:10.1093/toxsci/kfv230