High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts

GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of OLE1. U...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2005-05, Vol.99 (5), p.512-516
Main Authors: Yamada, Tasuku, Shimoi, Hitoshi, Ito, Kiyoshi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
BREWING
Fundamental and applied biological sciences. Psychology
GENES
OLE1
PCR
SAKE YEAST
unsaturated fatty acid
UNSATURATED FATTY ACIDS
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Summary:GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using OLE1 promoter:lacZ fusion reporter genes revealed that both the cis element of the OLE1 promoter and trans factors are involved in the increased expression of OLE1 in sake yeasts.
ISSN:1389-1723
1347-4421
DOI:10.1263/jbb.99.512